Gas chromatographic—mass spectrometric screening procedure for the identification of formaldehyde-derived tetrahydroisoquinolines in human urine
| Autor: | F. Musshoff, Th. Daldrup, W. Bonte |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Chromatography
Ethylamines Extraction (chemistry) Formaldehyde General Chemistry Isoquinolines Mass spectrometry Gas Chromatography-Mass Spectrometry Mass Spectrometry chemistry.chemical_compound chemistry Methyl chloroformate Tetrahydroisoquinolines Humans Sample preparation Gas chromatography Derivatization |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 619:235-242 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/0378-4347(93)80112-h |
| Popis: | A gas chromatographic—mass spectrometric method for the identification of 1,2,3,4-tetrahydro-β-carboline and four metabolites extracted from urine is described. In a first step the substances, formed by reaction of formaldehyde with biogenic amines, were derivatized in aqueous solution with methyl chloroformate to eliminate an artificial formation of these compounds via condensation of endogenous indole ethylamines with aldehydes or α-keto acids during the work-up procedure. This initial derivatization formed stable hydrophobic compounds and improved the extractability for a liquid—liquid extraction. Further clean-up was performed by solid-phase extraction on C 18 sample preparation columns. The method can identify these compounds in the picogram range. |
| Databáze: | OpenAIRE |
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