Use of next generation sequence to investigate potential novel macrolide resistance mechanisms in a population of Moraxella catarrhalis isolates
| Autor: | Xin-Xin Chen, Fanrong Kong, Meng Xiao, Ge Zhang, Yanping Gong, Jing-Wei Cheng, Timothy Kudinha, He-Ping Xu, Dongfang Li, Honglong Wu, Xin-Ying Wang, Yingchun Xu, Yinxin Zhang, Yali Liu, Li Zhang, Zhipeng Xu |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
DNA
Bacterial 0301 basic medicine 030106 microbiology Population Single-nucleotide polymorphism Microbial Sensitivity Tests Gene mutation Biology Article 03 medical and health sciences 23S ribosomal RNA Drug Resistance Bacterial Humans education Gene Genetics Comparative genomics education.field_of_study Multidisciplinary Ribosomal RNA Anti-Bacterial Agents RNA Ribosomal 23S 030104 developmental biology Mutation Multilocus sequence typing Macrolides Moraxella catarrhalis Multilocus Sequence Typing |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep35711 |
| Popis: | Although previous studies have confirmed that 23S rRNA gene mutation could be responsible for most of macrolide resistance in M. catarrhalis, a recent study suggested otherwise. Next generation sequence based comparative genomics has revolutionized the mining of potential novel drug resistant mechanisms. In this study, two pairs of resistant and susceptible M. catarrhalis isolates with different multilocus sequence types, were investigated for potential differential genes or informative single nucleotide polymorphisms (SNPs). The identified genes and SNPs were evaluated in 188 clinical isolates. From initially 12 selected differential genes and 12 informative SNPs, 10 differential genes (mboIA, mcbC, mcbI, mboIB, MCR_1794, MCR_1795, lgt2B/C, dpnI, mcbB, and mcbA) and 6 SNPs (C619T of rumA, T140C of rplF, G643A of MCR_0020, T270G of MCR_1465, C1348A of copB, and G238A of rrmA) were identified as possibly linked to macrolide resistance in M. catarrhalis. Most of the identified differential genes and SNPs are related to methylation of ribosomal RNA (rRNA) or DNA, especially MCR_0020 and rrmA. Further studies are needed to determine the function and/or evolution process, of the identified genes or SNPs, to establish whether some novel or combined mechanisms are truly involved in M. catarrhalis macrolide resistance mechanism. |
| Databáze: | OpenAIRE |
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