An evaluation of multiplex bead-based analysis of cytokines and soluble proteins in archived lithium heparin plasma, EDTA plasma and serum samples
| Autor: | Lise Saksø Mortensen, Brita Singers Sørensen, Simon Lønbro, Line Brøndum, Jens Overgaard, Jan Alsner, Jesper Grau Eriksen |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Eotaxin Adult Male Quality Control Vascular Endothelial Growth Factor A Analyte Clinical Biochemistry Edta plasma Specimen Handling Andrology 03 medical and health sciences 0302 clinical medicine medicine Humans Multiplex Edetic Acid Aged Aged 80 and over Cryopreservation Immunoassay Observer Variation Vascular Endothelial Growth Factor Receptor-1 medicine.diagnostic_test Chemistry Heparin Hepatocyte Growth Factor Leptin Anticoagulants Reproducibility of Results General Medicine Middle Aged Serum samples Vascular Endothelial Growth Factor Receptor-2 ErbB Receptors 030104 developmental biology Head and Neck Neoplasms 030220 oncology & carcinogenesis Case-Control Studies Immunology Cytokines Female medicine.drug |
| Zdroj: | Brøndum, L, Sørensen, B S, Eriksen, J G, Mortensen, L S, Lønbro, S, Overgaard, J & Alsner, J 2016, ' An evaluation of multiplex bead-based analysis of cytokines and soluble proteins in archived lithium heparin plasma, EDTA plasma and serum samples ', Scandinavian Journal of Clinical & Laboratory Investigation, vol. 76, no. 8, pp. 601-611 . https://doi.org/10.1080/00365513.2016.1230882 |
| DOI: | 10.1080/00365513.2016.1230882 |
| Popis: | Objective: To assess the usability of archived plasma and serum by multiplex (Luminex) analysis of circulating proteins (analytes) by evaluating the day to day variation, the effect of several freeze-thaw cycles, and the influence of the media and choice of anticoagulant. Methods: Nineteen analytes in plasma and serum from 86 head and neck cancer patients and 33 controls were evaluated: EGFR, leptin, OPN, VEGFR-1, VEGFR-2, IL-2, IL-13, PDGF-bb, TNF, PAI-1, SDF-1a, IL-4, IL-6, IL-8, eotaxin, G-CSF, VEGF, GRO-a, and HGF. Results: The correlation between measurements of the same samples analyzed on different dates was reasonable. However, samples run on different dates could exhibit different absolute values. The 75th percentile of the fold differences for samples run on different dates was 2.2. No significant difference was found between one and four freeze-thaw cycles (except for HGF), and the correlation was high. We found significant differences in mean concentrations of the majority of analytes in different media and with different anticoagulants. Only the following analytes did not show difference in mean concentrations: EDTA plasma vs. serum: leptin and VEGFR-2, LH plasma vs. serum: IL-2, IL-13, and VEGF, LH plasma levels vs. EDTA plasma: IL-2 and IL-4. Conclusion: Stored serum, LH plasma, and EDTA plasma from clinical trials can be used for analysis of circulating cytokines and proteins. Variations in measurements occur, but are within reasonable ranges. The optimal type of media depends on the analytes, as different analytes have low number of measurements below the lower limit of quantification and higher dynamic ranges in different media. |
| Databáze: | OpenAIRE |
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