MiRNA-124 is a link between measles virus persistent infection and cell division of human neuroblastoma cells
| Autor: | Christine M. Matullo, Isana Veksler-Lublinsky, Glenn F. Rall, Hila Naaman, Jacob Gopas, Yonat Shemer-Avni |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
RNA viruses Cell division lcsh:Medicine Apoptosis Pathology and Laboratory Medicine Biochemistry Neuroblastoma Medicine and Health Sciences Cell Cycle and Cell Division lcsh:Science Cultured Tumor Cells Multidisciplinary biology Cell Death Cell Differentiation 3. Good health Viral Persistence and Latency Nucleic acids Medical Microbiology Cell Processes Viral Pathogens Viruses Biological Cultures Pathogens Neuronal Differentiation Cell Division Research Article Programmed cell death Down-Regulation Measles Virus Transfection Research and Analysis Methods Microbiology Virus Measles virus 03 medical and health sciences Virology Cell Line Tumor microRNA Genetics Humans Non-coding RNA Molecular Biology Techniques Microbial Pathogens Molecular Biology Biology and life sciences lcsh:R Organisms Cell Biology Cyclin-Dependent Kinase 6 Cell Cultures biology.organism_classification Gene regulation MicroRNAs 030104 developmental biology Cell culture Paramyxoviruses biology.protein RNA Neuroblastoma Cells lcsh:Q Cyclin-dependent kinase 6 Gene expression Developmental Biology Measles |
| Zdroj: | PLoS ONE PLoS ONE, Vol 12, Iss 10, p e0187077 (2017) |
| ISSN: | 1932-6203 |
| Popis: | Measles virus (MV) infects a variety of lymphoid and non-lymphoid peripheral organs. However, in rare cases, the virus can persistently infect cells within the central nervous system. Although some of the factors that allow MV to persist are known, the contribution of host cell-encoded microRNAs (miRNA) have not been described. MiRNAs are a class of noncoding RNAs transcribed from genomes of all multicellular organisms and some viruses, which regulate gene expression in a sequence-specific manner. We have studied the contribution of host cell-encoded miRNAs to the establishment of MV persistent infection in human neuroblastoma cells. Persistent MV infection was accompanied by differences in the expression profile and levels of several host cell-encoded microRNAs as compared to uninfected cells. MV persistence infection of a human neuroblastoma cell line (UKF-NB-MV), exhibit high miRNA-124 expression, and reduced expression of cyclin dependent kinase 6 (CDK6), a known target of miRNA-124, resulting in slower cell division but not cell death. By contrast, acute MV infection of UKF-NB cells did not result in increased miRNA-124 levels or CDK6 reduction. Ectopic overexpression of miRNA-124 affected cell viability only in UKF-NB-MV cells, causing cell death; implying that miRNA-124 over expression can sensitize cells to death only in the presence of MV persistent infection. To determine if miRNA-124 directly contributes to the establishment of MV persistence, UKF-NB cells overexpressing miRNA-124 were acutely infected, resulting in establishment of persistently infected colonies. We propose that miRNA-124 triggers a CDK6-dependent decrease in cell proliferation, which facilitates the establishment of MV persistence in neuroblastoma cells. To our knowledge, this is the first report to describe the role of a specific miRNA in MV persistence. |
| Databáze: | OpenAIRE |
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