Polysaccharide-based vaccine delivery systems: Macromolecular assembly, interactions with antigen presenting cells, andin vivoimmunomonitoring

Autor: Alain Domard, Caroline Weber, Laurent David, Thierry Delair, Bernard Verrier, Marie-Hélène Charles, Alexandre Drogoz
Přispěvatelé: Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Ingénierie des Matériaux Polymères - Laboratoire des Matériaux Polymères et des Biomatériaux (IMP-LMPB), Centre National de la Recherche Scientifique (CNRS)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut de Chimie du CNRS (INC)
Rok vydání: 2009
Předmět:
02 engineering and technology
01 natural sciences
AQUEOUS-SOLUTION
GENE DELIVERY
Chitosan
Electrolytes
Mice
chemistry.chemical_compound
MICROSPHERES
Nanotechnology
Scattering
Radiation
DRUG-DELIVERY
polyelectrolyte complexes
AIDS Vaccines
Mice
Inbred BALB C
biology
IMMUNE-RESPONSES
CHITOSAN NANOPARTICLES
MICROPARTICLES
Vaccination
Metals and Alloys
[CHIM.MATE]Chemical Sciences/Material chemistry
Cell sorting
vaccine delivery
021001 nanoscience & nanotechnology
3. Good health
Macromolecular assembly
Drug delivery
Female
Antibody
0210 nano-technology
Materials science
Macromolecular Substances
SPHERICAL POLYELECTROLYTE BRUSHES
Biomedical Engineering
Antigen-Presenting Cells
Gene delivery
010402 general chemistry
DENDRITIC CELLS
Biomaterials
Antigen
Polysaccharides
Animals
Particle Size
Antigen-presenting cell
particles
HIV
Molecular biology
0104 chemical sciences
[CHIM.POLY]Chemical Sciences/Polymers
chemistry
Ceramics and Composites
biology.protein
Biophysics
Nanoparticles
chitosan
COMPLEX-FORMATION
Zdroj: Journal of Biomedical Materials Research Part A
Journal of Biomedical Materials Research Part A, Wiley, 2010, pp.1322-1334. ⟨10.1002/jbm.a.32605⟩
ISSN: 1552-4965
1549-3296
DOI: 10.1002/jbm.a.32605
Popis: International audience; Using a strategy of macromolecular assembly, a colloidal vaccine delivery system was obtained from chitosan and dextran sulfate and loaded with an antigenic protein (p24, the capsid protein of HIV-1). The colloidal polyelectrolyte complexes (PECs) were obtained by charge neutralization of the polyanion and polycation at a charge ratio (n(+)/n(-)) of 2 (CHDS). The conditions of assembly were tuned to maintain the colloidal properties of the carrier in high salt environment. The relative molar masses of the two polyions and the degree of acetylation (DA) of chitosan were essential parameters to achieve this goal, and this could be related to the nanometric scale organization of the colloids observed by Small Angle X-rays Scattering experiments. The binding of p24 to the colloidal carrier was achieved and the release of the antigen was investigated. Antigen presenting cells [dendritic cells (DCs)], obtained from monocytes, could internalize the colloids. Immature DCs (iDCs) were not matured by the colloidal PECs either loaded or not loaded with p24, as proved by Fluorescent Activated Cell Sorting (FACS) analysis. Despite this lack of in vitro interaction, a specific immune response was observed in mice with a high production of antibodies, after subcutaneous injection. The analysis of the interleukin production shows that both the cellular and the Immoral responses were stimulated. This work brings a physico-chemical insight on polysaccharide-based antigen delivery systems and opens up new perspectives for their use as vaccine carriers.
Databáze: OpenAIRE
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