Expanding the Reactive Sulfur Metabolome: Intracellular and Efflux Measurements of Small Oxoacids of Sulfur (SOS) and H2S in Human Primary Vascular Cell Culture

Autor:	Panagiotis Koutakis, Kristina Sorokolet, Ahmed Ismaeel, Ottis Scrivner, Patrick J. Farmer, Murugaeson R. Kumar
Rok vydání:	2021
Předmět:	hydrogen sulfide Pharmaceutical Science chemistry.chemical_element SOS (small oxoacids of sulfur) primary vascular cells hypoxia Analytical Chemistry QD241-441 Drug Discovery medicine Metabolome Physical and Theoretical Chemistry Oxidase test Chemistry Organic Chemistry equipment and supplies Sulfur Reverse electron flow Endothelial stem cell Mechanism of action Chemistry (miscellaneous) Cell culture Biophysics Molecular Medicine medicine.symptom Intracellular
Zdroj:	Molecules; Volume 26; Issue 23; Pages: 7160 Molecules, Vol 26, Iss 7160, p 7160 (2021)
ISSN:	1420-3049
DOI:	10.3390/molecules26237160
Popis:	Hydrogen sulfide (H2S) is an endogenous signaling molecule which is important for cardiovascular health, but its mechanism of action remains poorly understood. Here, we report measurements of H2S as well as its oxidized metabolites, termed small oxoacids of sulfur (SOS = HSOH and HOSOH), in four human primary vascular cell lines: smooth muscle and endothelial cells derived from both human arterial and coronary tissues. We use a methodology that targets small molecular weight sulfur species; mass spectrometric analysis allows for species quantification to report cellular concentrations based on an H2S calibration curve. The production of H2S and SOS is orders of magnitude higher in smooth muscle (nanomolar) as compared to endothelial cell lines (picomolar). In all the primary lines measured, the distributions of these three species were HOSOH >H2S > HSOH, with much higher SOS than seen previously in non-vascular cell lines. H2S and SOS were effluxed from smooth muscle cells in higher concentrations than endothelial cells. Aortic smooth muscle cells were used to examine changes under hypoxic growth conditions. Hypoxia caused notable increases in HSOH and ROS, which we attribute to enhanced sulfide quinone oxidase activity that results in reverse electron transport.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d25c0b8e47bdf6fe3a0a63b467449bfb https://doi.org/10.3390/molecules26237160 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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