Delayed hydrolysis of Raffinose Family Oligosaccharides (RFO) affects critical germination of chickpeas

Autor: Rex Arunraj, V. Kalaivani, Naskar Shoma, Raje Nikarika
Rok vydání: 2021
Předmět:
Zdroj: 3 Biotech
ISSN: 2190-5738
2190-572X
DOI: 10.1007/s13205-021-02764-1
Popis: Seed raffinose family oligosaccharides (RFOs) are converted into sucrose and galactose by α-galactosidase during germination. Seed osmopriming with a low concentration of potassium nitrate (KNO(3)) induces early and synchronized germination by activating hydrolases. Here, we report the effect of osmopriming on the germination indices of chickpea, its effects on α-galactosidase, and the fate of total RFOs. Chickpea seeds primed with 100 µM KNO(3) show early and synchronized germination but with reduced vigour after 48 h after imbibition (HAI) due to excess sucrose accumulation. The KNO(3) suppressed the activity of α-galactosidase during the imbibition stage that was later derepressed after 24 HAI, hence decreased the RFO levels accumulating high levels of sucrose after 48 HAI. The accumulated sucrose imposed a negative effect on the germination characteristics, particularly on seed vigour. Our results suggested that the sugar release and utilization were highly regulated and crucial during imbibition and germination; the enzyme α-galactosidase regulates sugar release from seed RFO reserve.
Databáze: OpenAIRE
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