Immunonutritional Protease Inhibitors from T. durum and A. sativa Display Metabolic Similarities When Assayed on Human Macrophage-like Cells
| Autor: | Aurora Garcia Tejedor, Bartosz Fotschki, Juan Antonio Nieto Fuentes, José Moisés Laparra Llopis |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
food.ingredient Avena Proteome QH301-705.5 medicine.medical_treatment Stimulation macrophage Article Catalysis Inorganic Chemistry 03 medical and health sciences 0302 clinical medicine food wheat medicine Humans Protease Inhibitors Glycolysis Physical and Theoretical Chemistry Biology (General) oat Molecular Biology immunonutrition QD1-999 Triticum Spectroscopy chemistry.chemical_classification Protease Molecular mass Plant Extracts Macrophages Organic Chemistry food and beverages General Medicine immunity Computer Science Applications Chemistry 030104 developmental biology Enzyme chemistry Biochemistry 030220 oncology & carcinogenesis Flux (metabolism) |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 8307, p 8307 (2021) International Journal of Molecular Sciences Volume 22 Issue 15 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | This study evaluated the immunonutritional effects caused by protease inhibitors from Avena sativa and Triticum durum to human macrophage-like cells. Macrophages were exposed (3 h) to extracts obtained from flours, and mitochondrial-associated oxygen consumption rates and inflammatory, metabolic, and proteome adaptations were quantified. Mass spectrometry ‘m/z’ signals of the extracts obtained from T. durum and A. sativa revealed molecular weights of 18–35 kDa and 16–22 kDa, respectively, for the compounds present at highest concentrations. Extracts from T. durum exhibited lower susceptibility to degradation by gastrointestinal enzymes than those from A. sativa: 9.5% vs 20.2%. Despite their different botanical origin, both extracts increased TLR4 expression. Metabolic protein levels were indicative of a decreased glycolytic to lactate flux in cell cultures upon stimulation with A. sativa extracts, which improved mitochondrial respiration in relation to those from T. durum. Principal components analysis confirmed relative similarities between immune–metabolic events triggered by immunonutritional ingredients in T. durum and A. sativa. Collectively, immunonutritional effects help to interpret the differences between both crops, worsening or improving, macrophage immune reactivity (tolerogenicity), and better control of inflammatory processes. |
| Databáze: | OpenAIRE |
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