The transcriptome of circulating sexually committed Plasmodium falciparum ring stage parasites forecasts malaria transmission potential

Autor: Courage Kakaney, Jones A. Amponsah, Festus K. Acquah, Kim C. Williamson, Elizabeth Cudjoe, Anwar E. Ahmed, Linda E. Amoah, James S. McCarthy, Michelle C. Barbeau, Ruth Ayanful-Torgby, Benjamin Abuaku, Evans K. Obboh, Surendra K Prajapati, Zuleima Pava
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Erythrocytes
Science
Plasmodium falciparum
030106 microbiology
Protozoan Proteins
General Physics and Astronomy
Gametogenesis
General Biochemistry
Genetics and Molecular Biology
Article
Transcriptome
03 medical and health sciences
Prognostic markers
parasitic diseases
Gametocyte
medicine
Animals
Humans
Parasite hosting
Malaria
Falciparum
Gene
health care economics and organizations
Life Cycle Stages
Multidisciplinary
biology
Transmission (medicine)
Gene Expression Profiling
Parasite genomics
Gene Expression Regulation
Developmental
Molecular Sequence Annotation
General Chemistry
social sciences
biology.organism_classification
medicine.disease
Virology
Parasite biology
Gene Ontology
030104 developmental biology
Transcription Factor AP-2
Carrier State
population characteristics
Ex vivo
Malaria
Biomarkers
Zdroj: Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Nature Communications
ISSN: 2041-1723
Popis: Malaria is spread by the transmission of sexual stage parasites, called gametocytes. However, with Plasmodium falciparum, gametocytes can only be detected in peripheral blood when they are mature and transmissible to a mosquito, which complicates control efforts. Here, we identify the set of genes overexpressed in patient blood samples with high levels of gametocyte-committed ring stage parasites. Expression of all 18 genes is regulated by transcription factor AP2-G, which is required for gametocytogenesis. We select three genes, not expressed in mature gametocytes, to develop as biomarkers. All three biomarkers we validate in vitro using 6 different parasite lines and develop an algorithm that predicts gametocyte production in ex vivo samples and volunteer infection studies. The biomarkers are also sensitive enough to monitor gametocyte production in asymptomatic P. falciparum carriers allowing early detection and treatment of infectious reservoirs, as well as the in vivo analysis of factors that modulate sexual conversion.
Malaria gametocytes are sexual-stage parasites transmitted from mammalian host’s blood back to their insect vector. Here, Prajapati et al. identify gametocyte-committed ring-stage biomarkers allowing to forecast malaria transmission potential.
Databáze: OpenAIRE
Externí odkaz: