Pregnancy outcomes for mouse preimplantation embryos exposed in vitro to the estrogenic pesticide o,p′-DDT
| Autor: | Tammy Ellis, Richard L. Berg, Anne R. Greenlee, Michael D. Mercieca |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Ratón Embryonic Development Apoptosis Mice Inbred Strains Biology Toxicology Morula DDT Mice Pregnancy Internal medicine medicine Animals Blastocyst Pesticides Cells Cultured Fetus Pregnancy Outcome Embryo Uterine horns Embryo Transfer medicine.disease In vitro Kinetics medicine.anatomical_structure Endocrinology Gestation Female |
| Zdroj: | Reproductive Toxicology. 20:229-238 |
| ISSN: | 0890-6238 |
| DOI: | 10.1016/j.reprotox.2005.02.004 |
| Popis: | Pregnancy outcomes were evaluated following uterine transfer of murine preimplantation embryos exposed in vitro to the estrogenic pesticide o , p ′-dichlorodiphenyltrichloroethane ( o , p ′-DDT). Single-cell embryos were incubated 72 h in medium droplets containing 0.1% ethanol (control) or 0.1 μg/ml o , p ′-DDT (pesticide). Morula and preblastocyst embryos were transferred in groups of eight to right uterine horns of pseudopregnant mice ( n = 111) and pups ( n = 132) were evaluated at Caesarean-section (C-section). In vitro exposure to o , p ′-DDT reduced development to morula ( P P = 0.05). However, treatment differences were not detected for implantation rates (35% versus 39%; P = 0.64), pup numbers per dam (2.3 versus 1.9; P = 0.36), transfer efficiencies (16% versus 14%; P = 0.53), fetal weights (1.56 g versus 1.57 g; P = 0.91), skeletal abnormalities (55% versus 66%; P = 0.47), or male ratios (54.8% versus 53.8%; P = 1.0). In vitro exposure of preimplantation embryos to 0.1 μg/ml o , p ′-DDT for 72 h resulted in no measurable effects on subsequent implantation or pup characteristics at C-section. |
| Databáze: | OpenAIRE |
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