P-selectin and intercellular adhesion molecule-1 expression after focal brain ischemia and reperfusion
| Autor: | E Mori, M M Tung, G. J. Del Zoppo, W. S. Thomas, Y. Okada, Brian Copeland |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Pathology medicine.medical_specialty Platelet Aggregation Endothelium P-selectin Intercellular Adhesion Molecule-1 Ischemia Platelet Membrane Glycoproteins Brain Ischemia Microcirculation Brain ischemia medicine Animals Advanced and Specialized Nursing Cell adhesion molecule business.industry Cerebral Arteries medicine.disease Immunohistochemistry Pathophysiology P-Selectin medicine.anatomical_structure Cerebrovascular Circulation Reperfusion Endothelium Vascular Neurology (clinical) Cardiology and Cardiovascular Medicine business Cell Adhesion Molecules Papio |
| Zdroj: | Stroke. 25:202-211 |
| ISSN: | 1524-4628 0039-2499 |
| DOI: | 10.1161/01.str.25.1.202 |
| Popis: | Polymorphonuclear leukocytes have been implicated in the development of the "no-reflow" phenomenon after focal cerebral ischemia and reperfusion. To further understand the role of granulocytes in microvascular occlusions, the responses of the granulocyte-endothelial cell adhesion molecules P-selectin and intercellular adhesion molecule-1 during middle cerebral artery ischemia and reperfusion were examined in a primate model. Twelve adolescent male baboons were used for 2-hour middle cerebral artery occlusion (n = 3) or for 3-hour occlusion with 1-hour (n = 3), 4-hour (n = 3), and 24-hour (n = 3) reperfusion, and three separate unoperated primates served as controls. A quantitative immunohistochemical study of the microvascular distribution of P-selectin and intercellular adhesion molecule-1 was performed using 10-microns frozen sections from basal ganglia analyzed with computerized light microscopy video imaging. Significant (P < .05) persistent upregulation of P-selectin (beginning during ischemia) and transient upregulation of intercellular adhesion molecule-1 (at 1 and 4 hours of reperfusion) were observed on endothelium of selected post-capillary microvessels of the ischemic lenticulostriate artery territory. Platelet accumulation also occurred in this territory and was responsible for a significant proportion of the nonendothelial P-selectin signal at 24 hours after reperfusion. Focal cerebral ischemia/reperfusion stimulates endothelial P-selectin and intercellular adhesion molecule-1 expression in brain microvessels in the ischemic zone, which may contribute to enhanced leukocyte adherence and persistent activation. |
| Databáze: | OpenAIRE |
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