Oxygen Consumption of Single Bovine Embryos Probed by Scanning Electrochemical Microscopy
| Autor: | Hitoshi Shiku, Hiroyuki Abe, Takuo Shiraishi, Hiroyoshi Hoshi, Hiroaki Ohya, Masato Kobayashi, Tornokazu Matsue |
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| Rok vydání: | 2001 |
| Předmět: |
Chemistry
Oxygene Analytical chemistry chemistry.chemical_element Embryo Microscopy Scanning Probe Embryo Mammalian Oxygen Analytical Chemistry Scanning electrochemical microscopy Oxygen Consumption medicine.anatomical_structure embryonic structures Electrochemistry medicine Animals Inner cell mass Cattle Limiting oxygen concentration Blastocyst computer reproductive and urinary physiology Embryo quality computer.programming_language |
| Zdroj: | Analytical Chemistry. 73:3751-3758 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/ac010339j |
| Popis: | Oxygen consumption of individual bovine embryos was noninvasively quantified by scanning electrochemical microscopy (SECM). A probe microelectrode was used to scan near a single embryo surface in a culture medium to monitor the oxygen reduction current at 37 degrees C, under a water-saturated atmosphere of 5% CO2 and 95% air. The oxygen concentration profiles near the embryos were in good agreement with the theoretical spherical diffusion. When an embryo reached the stage of a morula with a 74-microm radius on day 6 after in vitro fertilization, the oxygen concentration difference (deltaC) between the bulk solution and the morula surface was 6.90 +/- 1.35 microM. The oxygen consumption rate (F) of the single morula was estimated to be (1.40 +/- 0.27) x 10(-14) mol s(-1). After the SECM measurement, the embryo was continuously cultured for another 2 days and grew to the stage of a blastocyst with a 100-microm radius. For the blastocyst, the deltaC values for the inner cell mass side and the trophoblast side were 16.40 +/- 1.83 and 9.14 +/- 1.68 microM, respectively. The oxygen consumption rate of the blastocyst was found to be in the range of (2.50 +/- 0.46) x 10(-14) mol s(-1) < F < (4.49 +/- 0.50) x 10(-14) mol s(-1). We have carried out SECM measurements for 19 embryos, and the results were compared in detail with these from an optical microscopic observation. The deltaC values for the morulae on day 6 after in vitro fertilization were strongly related to the morphological embryo quality. The morulae showing a larger deltaC value developed into blastocysts of a larger size, and the deltaC value after the subsequent 2 days of cultivation was found to be increased. |
| Databáze: | OpenAIRE |
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