Isolation, Identification and Biotechnological Applications of a Novel, Robust, Free-living Chlorococcum (Oophila) amblystomatis Strain Isolated from a Local Pond
| Autor: | João Varela, Leonel Pereira, Hugo Pereira, Joana T. Silva, Nádia Abreu Correia, Lisa M. Schüler, M.R. Soares, Joana Silva, Tamára Santos, Carolina Bruno de Sousa, Margarida Costa |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Lutein
020209 energy Photobioreactor 02 engineering and technology Bioprospection lcsh:Technology photobioreactors lcsh:Chemistry Chlorococcum (Oophila) amblystomatis 03 medical and health sciences chemistry.chemical_compound Photobioreactors Dry weight Chlorococcum 0202 electrical engineering electronic engineering information engineering Microalgae General Materials Science Food science biotechnological applications lcsh:QH301-705.5 Instrumentation Carotenoid 030304 developmental biology Fluid Flow and Transfer Processes chemistry.chemical_classification Biotechnological applications 0303 health sciences Strain (chemistry) biology lcsh:T Process Chemistry and Technology microalgae General Engineering Ribosomal RNA biology.organism_classification bioprospection lcsh:QC1-999 Computer Science Applications lcsh:Biology (General) lcsh:QD1-999 chemistry lcsh:TA1-2040 Chlorophyll Industrial-scale production lcsh:Engineering (General). Civil engineering (General) lcsh:Physics industrial-scale production |
| Zdroj: | Applied Sciences Volume 10 Issue 9 Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Applied Sciences, Vol 10, Iss 3040, p 3040 (2020) |
| ISSN: | 2076-3417 |
| DOI: | 10.3390/app10093040 |
| Popis: | Bioprospection of novel autochthonous strains is key to the successful industrial-scale production of microalgal biomass. A novel Chlorococcum strain was recently isolated from a pond inside the industrial production facility of Allmicroalgae (Leiria, Portugal). Phylogenetic analysis based on 18S ribosomal ribonucleic acid (rRNA) gene sequences suggests that this isolate is a novel, free-living Oophila amblystomatis strain. However, as our phylogenetic data strongly suggests that the aforementioned taxon belongs to the genus Chlorococcum, it is here proposed to rename this species as Chlorococcum amblystomatis. In order to characterize the biotechnological potential of this novel isolate, growth performance and biochemical composition were evaluated from the pilot (2.5-m3) to industrial (10-m3) scale. The highest maximum areal productivity (36.56 g· m&minus 2· day&minus 1) was reached in a 10-m3 tubular photobioreactor (PBR), as compared to that obtained in a 2.5-m3 PBR (26.75 g· 1). Chlorococcum amblystomatis displayed high protein content (48%&ndash 56% dry weight (DW)) and moderate levels of total lipids (18%&ndash 31% DW), carbohydrates (6%&ndash 18% DW) and ashes (9%&ndash 16% DW). Furthermore, the lipid profile was dominated by polyunsaturated fatty acids (PUFAs). The highest pigment contents were obtained in the 2.5-m3 PBR, where total chlorophylls accounted for 40.24 mg· g&minus 1 DW, followed by lutein with 5.37 mg· 1 DW. Overall, this free-living Chlorococcum amblystomatis strain shows great potential for nutritional applications, coupling a promising growth performance with a high protein content as well as relevant amounts of PUFAs, chlorophyll, and carotenoids. |
| Databáze: | OpenAIRE |
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