Oxygenation of anandamide by lipoxygenases
| Autor: | Zadelhoff, G. van, Stelt, M. van der, Maccarrone, M. |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
0301 basic medicine
biology 2-Arachidonoylglycerol Anandamide Endocannabinoid system Serine Monoacylglycerol lipase 03 medical and health sciences chemistry.chemical_compound Lipoxygenase 030104 developmental biology 0302 clinical medicine chemistry Biochemistry Fatty acid amide hydrolase biology.protein lipids (amino acids peptides and proteins) Arachidonic acid 030217 neurology & neurosurgery |
| Zdroj: | Methods in Molecular Biology, 307-316. New York: Humana STARTPAGE=307;ENDPAGE=316;TITLE=Methods in Molecular Biology Methods in Molecular Biology ISBN: 9781071627273 Methods in Molecular Biology ISBN: 9781493935376 |
| Popis: | The endocannabinoids anandamide and 2-arachidonoylglycerol are not only metabolized by serine hydrolases, such as fatty acid amide hydrolase, monoacylglycerol lipase, and α,β-hydrolases 6 and 12, but they also serve as substrates for cyclooxygenases and lipoxygenases. These enzymes oxygenate the 1Z,4Z-pentadiene system of the arachidonic acid backbone of endocannabinoids, thereby giving rise to an entirely new array of bioactive lipids. Hereby, a protocol is provided for the enzymatic synthesis, purification, and characterization of various oxygenated metabolites of anandamide generated by lipoxygenases, which enables the biological study and detection of these metabolites. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|