Apolipoprotein A-I protection against atherosclerosis is dependent on genetic background
| Autor: | J. R. Lukens, Clarissa V. Bhanvadia, Paulette A. Krishack, Timothy J. Sontag, Godfrey S. Getz, Catherine A. Reardon |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Apolipoprotein E
Time Factors Apolipoprotein B Aortic Diseases Monocytes Cell Line Lesion chemistry.chemical_compound Mice Apolipoproteins E Species Specificity Plasma lipids medicine Animals Genetic Predisposition to Disease Aorta Brachiocephalic Trunk Triglycerides Mice Knockout biology Apolipoprotein A-I Cholesterol Strain (biology) Monocyte Macrophages Atherosclerosis Mice Inbred C57BL Disease Models Animal medicine.anatomical_structure chemistry Immunology biology.protein lipids (amino acids peptides and proteins) Female medicine.symptom Cardiology and Cardiovascular Medicine Lipoprotein |
| Zdroj: | Arteriosclerosis, thrombosis, and vascular biology. 34(2) |
| ISSN: | 1524-4636 |
| Popis: | Objective— Inbred mouse strains have different susceptibilities to experimental atherosclerosis. The C57BL/6 strain is among the most sensitive and has, therefore, been the most widely used in atherosclerosis studies, whereas many strains are resistant. The FVB/N strain is highly resistant to atherosclerosis on the apolipoprotein E (apoE)– and low-density lipoprotein (LDL) receptor–deficient backgrounds. High-density lipoprotein and its major apoprotein, apoA-I, have been shown to be protective against atherogenesis on the C57BL/6 background. We here examine the influence of genetic background on the atheroprotective nature of apoA-I. Approach and Results— ApoE-deficient/apoA-I-deficient mice were generated in the C57BL/6 and FVB/N strains from apoE-deficient mice. After 6 to 10 weeks on a Western-type diet, plasma lipids and atherosclerotic lesion size were assessed. Macrophage recruitment, cholesterol regulation, and blood monocyte levels were examined as potential mechanisms driving lesion size differences. FVB/N knockout mice had higher plasma very-LDL/LDL cholesterol than their C57BL/6 counterparts. ApoA-I deficiency decreased very-LDL/LDL cholesterol in C57BL/6 mice but not in FVB/N mice. FVB/N single and double knockout mice had less lesion than C57BL/6 6 to 10 weeks on diet. ApoA-I deficiency augmented lesion development only in C57BL/6 mice. Macrophage recruitment to thioglycollate-treated peritoneum and diet-induced blood monocyte levels reflected the pattern of lesion development among the 4 genotypes. ApoA-I deficiency increased macrophage cholesterol content only in C57BL/6. FVB/N plasma was a better acceptor for macrophage cholesterol efflux than C57BL/6. Conclusions— ApoA-I is atheroprotective only in certain genetic contexts. In the C57BL/6 context, but not FVB/N, apoA-I decreases inflammatory macrophage recruitment and monocytosis, contributors to lesion formation. |
| Databáze: | OpenAIRE |
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