High-density, long-lasting, and multi-region electrophysiological recordings using polymer electrode arrays
| Autor: | Jeremy F. Magland, Jeanine A. Pebbles, Angela C. Tooker, Kye Y Lee, Loren M. Frank, Leslie Greengard, Charlotte Geaghan-Breiner, Jason E. Chung, Magnus Karlsson, Vanessa Tolosa, Supin Chen, Hannah R. Joo, Jiang Lan Fan, Mattias P. Karlsson, Alex H. Barnett, Daniel F. Liu, Hexin Liang |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Brain activity and meditation Computer science Polymers Stability (probability) 03 medical and health sciences 0302 clinical medicine Biological neural network Animals Rats Long-Evans Millisecond business.industry General Neuroscience Brain Polymer electrode Ranging Modular design Electrodes Implanted Electrophysiological Phenomena Rats Electrophysiology 030104 developmental biology Spike sorting Neuronal circuits Temporal resolution Nerve Net business Neuroscience 030217 neurology & neurosurgery Computer hardware |
| DOI: | 10.1101/242693 |
| Popis: | The brain is a massively interconnected neuronal network, organized into specialized circuits consisting of large ensembles of neurons distributed across anatomically connected regions. While circuit computations depend upon millisecond timescale interactions, the structure of the underlying networks are remodeled on timescales ranging from seconds to months. Current approaches lack the combination of resolution, spatial coverage, longevity, and stability to measure the detailed dynamics of these networks. Here we describe a large-scale, multisite recording platform that integrates polymer electrodes with a modular stacking headstage design supporting up to 1024 channels of recording in freely-behaving rats. We show that the integrated system can yield months-long recordings from hundreds of well-isolated units across multiple regions. Moreover, the recordings are stable enough to track a substantial fraction of single units for over a week. This platform enables large-scale electrophysiological interrogation of the function and evolution of distributed circuits throughout an animal9s adult life. |
| Databáze: | OpenAIRE |
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