The use of whole-genome sequencing for molecular epidemiology and antimicrobial surveillance: identifying the role of IncX3 plasmids and the spread of blaNDM-4-like genes in the Enterobacteriaceae
| Autor: | Björn A. Espedido, Sebastiaan J. van Hal, Slade O. Jensen, Borce Dimitrijovski |
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| Rok vydání: | 2015 |
| Předmět: |
DNA
Bacterial medicine.medical_specialty Prosthesis-Related Infections Genotype Context (language use) Myanmar Biology beta-Lactamases Pathology and Forensic Medicine Plasmid Antibiotic resistance Predictive Value of Tests Drug Resistance Multiple Bacterial Molecular genetics medicine Humans Gene Whole genome sequencing Genetics Molecular Epidemiology Travel Molecular epidemiology Australia Endocarditis Bacterial General Medicine Anti-Bacterial Agents Bacterial Typing Techniques Klebsiella Infections Resistome Klebsiella pneumoniae Phenotype Heart Valve Prosthesis Genome-Wide Association Study Plasmids |
| Zdroj: | Journal of Clinical Pathology. 68:835-838 |
| ISSN: | 1472-4146 0021-9746 |
| DOI: | 10.1136/jclinpath-2015-203044 |
| Popis: | AimsTo characterise the resistome of a multi-drug resistant Klebsiella pneumoniae (Kp0003) isolated from an Australian traveller who was repatriated to a Sydney Metropolitan Hospital from Myanmar with possible prosthetic aortic valve infective endocarditis.MethodsKp0003 was recovered from a blood culture of the patient and whole genome sequencing was performed. Read mapping and de novo assembly of reads facilitated in silico multi-locus sequence and plasmid replicon typing as well as the characterisation of antibiotic resistance genes and their genetic context. Conjugation experiments were also performed to assess the plasmid (and resistance gene) transferability and the effect on the antibiotic resistance phenotype.ResultsImportantly, and of particular concern, the carbapenem-hydrolysing β-lactamase gene blaNDM-4 was identified on a conjugative IncX3 plasmid (pJEG027). In this respect, the blaNDM-4 genetic context is similar (at least to some extent) to what has previously been identified for blaNDM-1 and blaNDM-4-like variants.ConclusionsThis study highlights the potential role that IncX3 plasmids have played in the emergence and dissemination of blaNDM-4-like variants worldwide and emphasises the importance of resistance gene surveillance. |
| Databáze: | OpenAIRE |
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