Expression of WNT1 in ameloblastoma and its significance
| Autor: | Xiu‑Qiu Gao, Jia Ji, Guan‑Nan Wang, Tian‑Fu Wang, Ming Zhong, Yv Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research animal structures WNT1 ameloblastoma 03 medical and health sciences 0302 clinical medicine Western blot Gene expression medicine Ameloblastoma western blot Oncogene medicine.diagnostic_test Chemistry Articles medicine.disease Molecular medicine Molecular biology Blot 030104 developmental biology Oncology 030220 oncology & carcinogenesis embryonic structures immunohistochemistry Immunohistochemistry Keratocystic Odontogenic Tumor |
| Zdroj: | Oncology Letters |
| ISSN: | 1792-1082 1792-1074 |
| Popis: | The present study aimed to measure the expression of WNT1 in ameloblastoma (AB). Immunohistochemistry was used to observe changes in WNT1 expression in 80 AB samples, 10 keratocystic odontogenic tumor (KCOT) samples and 10 normal oral mucosa (NOM) samples. Western blotting and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) were used to measure WNT1 protein and mRNA expression, respectively, in 30 AB samples, 5 KCOT samples, 5 NOM samples and 3 tooth germ samples. Ectopic cytoplasmic expression of WNT1 was detected in AB; 88.8% (71/80) of the samples were WNT1-positive. The western blotting results demonstrated that compared with NOM (0.57±0.05), WNT1 expression was significantly higher in AB tissue (1.74±0.36, P0.05). RT-qPCR revealed that the level of WNT1 gene expression in AB was increased 2.43-fold compared with normal mucosa, and 1.77-fold compared with tooth germ tissue. In conclusion, WNT1 protein and mRNA expression were increased in AB, and there was ectopic cytoplasmic expression. This indicates that WNT1 may serve an important role in AB occurrence and development. |
| Databáze: | OpenAIRE |
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