HepG2 Cells Expressing MicroRNA miR-122 Support the Entire Hepatitis C Virus Life Cycle ▿
| Autor: | Matthew J. Evans, Gusti M. Zeiner, Benjamin Israelow, Christopher M. Narbus, Marion Sourisseau, Maria L. Michta, Sharon E. Hopcraft |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Hepatitis C virus
Hepacivirus Immunology Gene Expression medicine.disease_cause Virus Replication Microbiology Viral entry Virology microRNA Gene expression MiR-122 medicine Humans Virus Release biology virus diseases Hep G2 Cells Virus Internalization biology.organism_classification digestive system diseases Virus-Cell Interactions NS2-3 protease MicroRNAs Viral replication Insect Science Hepatocytes |
| Popis: | The liver-specific microRNA miR-122 is required for efficient hepatitis C virus (HCV) RNA replication both in cell culture and in vivo . In addition, nonhepatic cells have been rendered more efficient at supporting this stage of the HCV life cycle by miR-122 expression. This study investigated how miR-122 influences HCV replication in the miR-122-deficient HepG2 cell line. Expression of this microRNA in HepG2 cells permitted efficient HCV RNA replication and infectious virion production. When a missing HCV receptor is also expressed, these cells efficiently support viral entry and thus the entire HCV life cycle. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|