Surface biotinylation of cytotoxic T lymphocytes for in vivo tracking of tumor immunotherapy in murine models
| Autor: | Zhenwei Yao, Benjamin Pulli, Grant K. Lewandrowski, Bakhos A. Tannous, John W. Chen, Anning Li, Jenny J. Linnoila, Xiaoyuan Feng, Matthias W.G. Zeller, Yue Wu, Muhammad Ali, Benoit Tricot, Edmund J. Keliher, Jing-Hui Li, Giulia Fulci, Gregory R. Wojtkiewicz, Cuihua Wang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research medicine.medical_treatment Immunology Biology Article Mice 03 medical and health sciences 0302 clinical medicine In vivo Glioma medicine Animals Humans Immunology and Allergy Cytotoxic T cell Biotinylation Viability assay Cell growth Immunotherapy medicine.disease Disease Models Animal CTL 030104 developmental biology Oncology 030220 oncology & carcinogenesis Cancer research Female CD8 T-Lymphocytes Cytotoxic |
| Zdroj: | Cancer Immunology, Immunotherapy. 65:1545-1554 |
| ISSN: | 1432-0851 0340-7004 |
| DOI: | 10.1007/s00262-016-1911-9 |
| Popis: | Currently, there is no stable and flexible method to label and track cytotoxic T-lymphocytes (CTLs) in vivo in CTL immunotherapy. We aimed to evaluate whether the sulfo-hydroxysuccinimide (NHS)-biotin-streptavidin (SA) platform could chemically modify the cell surface of CTLs for in vivo tracking. CD8+ T lymphocytes were labeled with sulfo-NHS-biotin under different conditions and then incubated with SA-Alexa647. Labeling efficiency was proportional to sulfo-NHS-biotin concentration. CD8+ T lymphocytes could be labeled with higher efficiency with sulfo-NHS-biotin in DPBS than in RPMI (P |
| Databáze: | OpenAIRE |
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