Actin dynamics coupled to clathrin-coated vesicle formation at the trans-Golgi network
| Autor: | Claire X. Zhang, Sébastien Carréno, David G. Drubin, Åsa E. Y. Engqvist-Goldstein, Kent L. McDonald |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Endocytic cycle
Proto-Oncogene Proteins pp60(c-src) Arp2/3 complex macromolecular substances Clathrin Cathepsin D 03 medical and health sciences symbols.namesake 0302 clinical medicine Lysosome Report Cell cortex medicine Image Processing Computer-Assisted TGN lysosomes clathrin actin Hip1R Humans Actin-binding protein RNA Small Interfering Fluorescent Antibody Technique Indirect 030304 developmental biology Adaptor Proteins Signal Transducing 0303 health sciences biology Actin remodeling Clathrin-Coated Vesicles Cell Biology Golgi apparatus Actins Cell biology medicine.anatomical_structure biology.protein symbols Lysosomes 030217 neurology & neurosurgery HeLa Cells Protein Binding trans-Golgi Network |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | In diverse species, actin assembly facilitates clathrin-coated vesicle (CCV) formation during endocytosis. This role might be an adaptation specific to the unique environment at the cell cortex, or it might be fundamental, facilitating CCV formation on different membranes. Proteins of the Sla2p/Hip1R family bind to actin and clathrin at endocytic sites in yeast and mammals. We hypothesized that Hip1R might also coordinate actin assembly with clathrin budding at the trans-Golgi network (TGN). Using deconvolution and time-lapse microscopy, we showed that Hip1R is present on CCVs emerging from the TGN. These vesicles contain the mannose 6-phosphate receptor involved in targeting proteins to the lysosome, and the actin nucleating Arp2/3 complex. Silencing of Hip1R expression by RNAi resulted in disruption of Golgi organization and accumulation of F-actin structures associated with CCVs on the TGN. Hip1R silencing and actin poisons slowed cathepsin D exit from the TGN. These studies establish roles for Hip1R and actin in CCV budding from the TGN for lysosome biogenesis. |
| Databáze: | OpenAIRE |
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