Profiling of Dehydropyrrolizidine Alkaloids and their N-Oxides in Herbarium-Preserved Specimens of Amsinckia Species Using HPLC-esi(+)MS
| Autor: | Kip E. Panter, Stanley L. Welsh, Joseph M. Betz, Dale R. Gardner, Steven M. Colegate |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization herbarium samples Natural variation lycopsamine Amsinckia Article dehydropyrrolizidine alkaloids Tandem Mass Spectrometry Botany MS/MS Chromatography High Pressure Liquid Pyrrolizidine Alkaloids Intermedine Chromatography biology General Chemistry Boraginaceae Amsinckia species HPLC-esiMS biology.organism_classification Plant Leaves Lycopsamine Herbarium Hplc esi ms intermedine General Agricultural and Biological Sciences |
| Zdroj: | Journal of Agricultural and Food Chemistry |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf500425v |
| Popis: | Species of the Amsinckia genus (Boraginaceae) are known to produce potentially hepato-, pneumo-, and/or genotoxic dehydropyrrolizidine alkaloids. However, the taxonomic differentiation of Amsinckia species can be very subtle and there seems to be marked differences in toxicity toward grazing livestock. Methanol extracts of mass-limited leaf samples from herbarium specimens (collected from 1899 to 2013) of 10 Amsinckia species and one variety were analyzed using HPLC-esi(+)MS and MS/MS for the presence of potentially toxic dehydropyrrolizidine alkaloids and/or their N-oxides. Dehydropyrrolizidine alkaloids were detected in all specimens examined ranging from about 1 to 4000 μg/g of plant. Usually occurring mainly as their N-oxides, the predominant alkaloids were the epimeric lycopsamine and intermedine. Also sometimes observed in higher concentrations were the 3'- and 7-acetyl derivatives of lycopsamine/intermedine and their N-oxides. Within a designated species, an inconsistent profile was often observed that may be due to natural variation, taxonomic misassignment, or nonuniform degradation due to plant collection and storage differences. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|