Pepino mosaic virus RNA-Dependent RNA Polymerase POL Domain Is a Hypersensitive Response-Like Elicitor Shared by Necrotic and Mild Isolates
| Autor: | Cristina Gómez-Aix, Fabiola Ruiz-Ramón, Miguel A. Aranda, Beata Hasiów-Jaroszewska, Pedro Gómez, M.A. Sanchez-Pina, Raquel Navarro Sempere |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Hypersensitive response Necrosis Genotype viruses Molecular Sequence Data education RNA-dependent RNA polymerase Nicotiana benthamiana Plant Science Cyclopentanes Environment 01 natural sciences Virus 03 medical and health sciences chemistry.chemical_compound Solanum lycopersicum Plant Growth Regulators RNA polymerase Tobacco medicine Amino Acid Sequence Oxylipins Polymerase Plant Diseases biology food and beverages Potexvirus biology.organism_classification RNA-Dependent RNA Polymerase Virology Protein Structure Tertiary Plant Leaves 030104 developmental biology chemistry Host-Pathogen Interactions biology.protein medicine.symptom Salicylic Acid Agronomy and Crop Science Sequence Alignment 010606 plant biology & botany |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Pepino mosaic virus (PepMV) is an emerging pathogen that represents a serious threat to tomato production worldwide. PepMV-induced diseases manifest with a wide range of symptoms, including systemic necrosis. Our results showed that PepMV accumulation depends on the virus isolate, tomato cultivar, and environmental conditions, and associates with the development of necrosis. Substitution of lysine for glutamic acid at position 67 in the triple gene block 3 (TGB3) protein, previously described as a necrosis determinant, led to increased virus accumulation and was necessary but not sufficient to induce systemic necrosis. Systemic necrosis both in tomato and Nicotiana benthamiana shared hypersensitive response (HR) features, allowing the assessment of the role of different genomic regions on necrosis induction. Overexpression of both TGB3 and the polymerase domain (POL) of the RNA-dependent RNA polymerase (RdRp) resulted in necrosis, although only local expression of POL triggered HR-like symptoms. Our results also indicated that the necrosis-eliciting activity of POL resides in its highly conserved “palm” domain, and that necrosis was jasmonic acid-dependent but not salicylic acid-dependent. Altogether, our data suggest that the RdRp-POL domain plays an important role in PepMV necrosis induction, with necrosis development depending on the virus accumulation level, which can be modulated by the nature of TGB3, host genotype and environmental conditions. |
| Databáze: | OpenAIRE |
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