Biomimetic Three Dimensional Cell Culturing: Colorectal Cancer Micro-Tissue Engineering
| Autor: | Yusra Kassim, J r me Couteau, Didier Lecerf, Elise Demange, Thomas Simon, Elias Al Tawil, Jean Pierre Vannier, Catherine Buquet |
|---|---|
| Přispěvatelé: | Micro-Environnement et Régulation Cellulaire Intégrée (MERCI), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Polymères Biopolymères Surfaces (PBS), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), TOXEM, CENELYS |
| Rok vydání: | 2014 |
| Předmět: |
Pathology
medicine.medical_specialty Micro-tissue Extracellular matrix 03 medical and health sciences 3D cell culture chemistry.chemical_compound 0302 clinical medicine Tissue engineering In vivo Hyaluronic acid medicine Hyaluronan 030304 developmental biology 0303 health sciences Cell growth business.industry [CHIM.MATE]Chemical Sciences/Material chemistry Cell cycle Colorectal cancer Cell biology Hydrogel [CHIM.POLY]Chemical Sciences/Polymers chemistry Cell culture 030220 oncology & carcinogenesis business |
| Zdroj: | Journal of Clinical & Experimental Oncology Journal of Clinical & Experimental Oncology, SciTechnol, 2014, 03 (02), ⟨10.4172/2324-9110.1000123⟩ |
| ISSN: | 2324-9110 |
| DOI: | 10.4172/2324-9110.1000123 |
| Popis: | International audience; Background: Spheroid cultures are known to mimic closely the properties of tumor tissue than monolayer cultures with regard to growth kinetics and metabolic rates. The aim of this paper is to confirm that tumor micro-tissue in a 3D biocompatible microenvironment maintain the cells natural behavior when compared to 2D monolayer culturing.Method: In order to validate our 3D culture system, we compared the 3D culture within a cross-linked hydrogel of hyaluronic acid, one of the major components of the extracellular matrix and the conventional 2D culture system.Results: Interestingly within our culture system, cells could be analyzed either after retrieval from the scaffold or even without being extracted in the 3D form rendering the HA hydrogel an ideal tool for biological applications. We observed the difference in the cell cycle, cell proliferation and behavior in both culture systems. Additionally drug testing was carried out using a chemotherapeutic agent (cis-platinium) that is already in clinical use to unequivocally prove the clinical predictive significance of the test strategy as compared with less complex assay systems and more complex in vivo models. We observed the presence of cell cycle heterogeneity very similar to the situation in vivo human tumors. Moreover, we have confirmed that resistance to chemotherapeutic reagents within this 3D culture system is much higher than those used in 2D cultures, since the tight assembly of cells in 3D culture systems render them more resistant requiring chemotherapeutic doses that recapitulate the drug sensitivity of tumor cells in vivo. Additionally we have observed the difference of apoptotic protein expression between 2D and 3D cell culture. |
| Databáze: | OpenAIRE |
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