The role of chromophore in the lipid-protein interactions in bacteriorhodopsin-phosphatidylcholine vesicles
| Autor: | Kazuo Yoshihara, Krzysztof Bryl |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Diphenylhexatriene
Phase transition Biophysics Bacteriorhodopsin Photochemistry Biochemistry Fluorescence excitation spectrum chemistry.chemical_compound Chromophore Structural Biology Phosphatidylcholine Phase (matter) Genetics Lipid phase transition Lipid bilayer phase behavior Molecular Biology Fluorescent Dyes biology Chemistry Vesicle Lipid–protein interaction Cell Biology Lipid Metabolism Glycerylphosphorylcholine Bacteriorhodopsins biology.protein lipids (amino acids peptides and proteins) |
| Zdroj: | FEBS Letters. 480:123-126 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(00)01910-4 |
| Popis: | By fluorescence and phase properties of a 1-acyl-2-[8-(2-anthroyl)-octanoyl]-sn-glycero-3-phosphocholine probe, the influence of the chromophore on the phase transition of bacteriorhodopsin–lipid vesicles was investigated. It was observed that removal of the chromophore led to the down-shifting of the phase transition temperatures. The temperatures corresponding to the beginning and ending of the gel–liquid phase transition were also influenced. This demonstrated that the liquid phase is reached more easily when the chromophore is bleached. The results indicate that removal of the chromophore alters the protein–lipid interactions. It is suggested that this alteration might be related to the change in the lipid molecular packing. |
| Databáze: | OpenAIRE |
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