Rapid species diagnosis for invasive candidiasis using mass spectrometry
| Autor: | Ralitsa Atanasova, Laura Djamdjian, Jean-Louis Golmard, Pierre Buffet, Arnaud Fekkar, Isabelle Meyer, Jean-Yves Brossas, Annick Datry, Carine Marinach-Patrice, Johanna Gomes, Georges Snounou, Christophe Hennequin, Dominique Mazier |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Proteomics
lcsh:Medicine Biology Bioinformatics Mass spectrometry Microbiology Infectious Diseases/Fungal Infections medicine Humans Candida albicans lcsh:Science Fungemia Multidisciplinary lcsh:R Candidiasis Microbiology/Medical Microbiology biology.organism_classification medicine.disease Blood proteins Yeast Biophysics/Protein Chemistry and Proteomics Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization lcsh:Q Subculture (biology) Time-of-flight mass spectrometry Research Article |
| Zdroj: | PLoS ONE, Vol 5, Iss 1, p e8862 (2010) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Background Matrix-assisted laser desorption ionisation time of flight mass spectrometry (MALDI TOF-MS) allows the identification of most bacteria and an increasing number of fungi. The potential for the highest clinical benefit of such methods would be in severe acute infections that require prompt treatment adapted to the infecting species. Our objective was to determine whether yeasts could be identified directly from a positive blood culture, avoiding the 1–3 days subculture step currently required before any therapeutic adjustments can be made. Methodology/Principal Findings Using human blood spiked with Candida albicans to simulate blood cultures, we optimized protocols to obtain MALDI TOF-MS fingerprints where signals from blood proteins are reduced. Simulated cultures elaborated using a set of 12 strains belonging to 6 different species were then tested. Quantifiable spectral differences in the 5000–7400 Da mass range allowed to discriminate between these species and to build a reference database. The validation of the method and the statistical approach to spectral analysis were conducted using individual simulated blood cultures of 36 additional strains (six for each species). Correct identification of the species of these strains was obtained. Conclusions/Significance Direct MALDI TOF-MS analysis of aliquots from positive blood cultures allowed rapid and accurate identification of the main Candida species, thus obviating the need for sub-culturing on specific media. Subsequent to this proof-of-principle demonstration, the method can be extended to other clinically relevant yeast species, and applied to an adequate number of clinical samples in order to establish its potential to improve antimicrobial management of patients with fungemia. |
| Databáze: | OpenAIRE |
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