HDL2 of heavy alcohol drinkers enhances cholesterol efflux from raw macrophages via phospholipid-rich HDL 2b particles
| Autor: | Markku J. Savolainen, Jari Metso, Minna L. Hannuksela, Sanna Mäkelä, Tiina M. Lehto, Mika Ala-Korpela, Matti Jauhiainen |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Male medicine.medical_specialty Alcohol Drinking Phospholipid Medicine (miscellaneous) Alcohol High-Density Lipoproteins Pre-beta Toxicology Cell Line Phosphatidylcholine-Sterol O-Acyltransferase chemistry.chemical_compound Mice Internal medicine Cholesterylester transfer protein medicine Distribution (pharmacology) Animals Humans Phospholipid Transfer Proteins Ethanol biology Cholesterol Macrophages Reverse cholesterol transport Cholesterol HDL Lipoproteins HDL3 Middle Aged Lipoproteins HDL2 Cholesterol Ester Transfer Proteins Psychiatry and Mental health Alcoholism Endocrinology Biochemistry chemistry biology.protein lipids (amino acids peptides and proteins) Efflux Lipoproteins HDL |
| Zdroj: | Alcoholism, clinical and experimental research. 32(6) |
| ISSN: | 1530-0277 |
| Popis: | Background: Alcohol consumption is associated with increased serum high density lipoprotein (HDL) cholesterol levels and a decreased risk for the development of atherosclerosis. However, the effects of heavy alcohol intake on reverse cholesterol transport, one of the key anti-atherogenic processes related to HDL, are poorly known. Methods: The ability of total HDL as well as HDL2 and HDL3 subclasses to promote cholesterol efflux from 3H-cholesterol-labeled RAW 264.7 macrophages was studied among 6 heavy alcohol drinkers and 6 controls. Distribution of HDL subclasses was analyzed by 4 to 30% native gradient gels. Serum phospholipid transfer protein (PLTP) and cholesteryl ester transfer protein (CETP) activities were analyzed among several other biochemical measures. Results: Cholesterol efflux to HDL2 of heavy drinkers was 22% (p = 0.025) higher relative to controls. The increase in HDL2 phospholipids, with a concomitant 2-fold (p = 0.055) increase in large HDL2b particles, was associated with enhanced cholesterol efflux to HDL2. Interestingly, the cholesterol efflux to HDL3 did not differ between the 2 study groups. These findings may be partially explained by a decreased CETP activity (−26%, p = 0.037) and an increased PLTP activity (39%, p = 0.045) in heavy drinkers. Conclusions: The increased cholesterol efflux potential of HDL2 is most likely an anti-atherogenic feature linked to heavy alcohol consumption. The cholesterol efflux and HDL phospholipids also associated strongly within the whole study group (rs = 0.910, p ≤ 0.01) suggesting a common pathway of enhanced cholesterol efflux via enlarged phospholipid-rich HDL particles. |
| Databáze: | OpenAIRE |
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