Spatial and temporal tracking of cardiac exosomes in mouse using a nano-luciferase-CD63 fusion protein

Autor: Jiang Chang, Weijia Luo, Xiaojing Yue, Kelsey C. Andrade-Powell, Zhishi Chen, Yuan Dai
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Genetically modified mouse
Male
Cell biology
RNA
Untranslated
Time Factors
Recombinant Fusion Proteins
Medicine (miscellaneous)
Endogeny
030204 cardiovascular system & hematology
Biology
Exosomes
Exosome
General Biochemistry
Genetics and Molecular Biology
Article
Animals
Genetically Modified
03 medical and health sciences
0302 clinical medicine
Live cell imaging
Bioluminescence
Animals
Luciferase
Myocytes
Cardiac
Tissue Distribution
Luciferases
Promoter Regions
Genetic
lcsh:QH301-705.5
Cells
Cultured
Biological models
Integrases
Myosin Heavy Chains
Tetraspanin 30
Fibroblasts
Fusion protein
Microvesicles
Cardiovascular biology
Mice
Inbred C57BL
030104 developmental biology
lcsh:Biology (General)
Luminescent Measurements
Extracellular signalling molecules
General Agricultural and Biological Sciences
Zdroj: Communications Biology, Vol 3, Iss 1, Pp 1-9 (2020)
Communications Biology
ISSN: 2399-3642
DOI: 10.1038/s42003-020-0830-7
Popis: Exosomes are secreted extracellular vesicles with lipid bilayer membranes. They are emerging as a new category of messengers that facilitate cross-talk between cells, tissues, and organs. Thus, a critical demand arises for the development of a sensitive and non-invasive tracking system for endogenous exosomes. We have generated a genetic mouse model that meets this goal. The Nano-luciferase (NanoLuc) reporter was fused with the exosome surface marker CD63 for exosome labeling. The cardiomyocyte-specific αMHC promoter followed by the loxP-STOP-loxP cassette was engineered for temporal and spatial labeling of exosomes originated from cardiomyocytes. The transgenic mouse was bred with a tamoxifen-inducible Cre mouse (Rosa26Cre-ERT2) to achieve inducible expression of CD63NanoLuc reporter. The specific labeling and tissue distribution of endogenous exosomes released from cardiomyocytes were demonstrated by luciferase assay and non-invasive bioluminescent live imaging. This endogenous exosome tracking mouse provides a useful tool for a range of research applications.
Using nano-luciferase-CD63, Weijia Luo et al. develop transgenic mice where cardiac exosomes can be tracked with non-invasive bioluminescent live imaging in a tamoxifen-inducible manner. These mice serve as a valuable tool that allow researchers to track cardiac exosomes in a defined time window.
Databáze: OpenAIRE
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