Excess Folic Acid Increases Lipid Storage, Weight Gain, and Adipose Tissue Inflammation in High Fat Diet-Fed Rats
Autor: | Marta Ordoñez, René L. Jacobs, Randal Nelson, Sally P. Stabler, Kelly Leonard, Catherine J. Field, John P. Kennelly, Antonio Gómez-Muñoz, Karen B. Kelly |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Blood Glucose medicine.medical_specialty obesity folic acid metabolic syndrome adipose tissue Adipose tissue lcsh:TX341-641 Biology Weight Gain Article Impaired glucose tolerance 03 medical and health sciences chemistry.chemical_compound Mice Internal medicine 3T3-L1 Cells medicine Animals RNA Messenger 2. Zero hunger Inflammation 030109 nutrition & dietetics Nutrition and Dietetics Triglyceride food and beverages Lipid metabolism Metabolism medicine.disease Lipid Metabolism Obesity Dietary Fats Rats 030104 developmental biology Endocrinology chemistry medicine.symptom Metabolic syndrome lcsh:Nutrition. Foods and food supply Weight gain Food Science |
Zdroj: | Nutrients Nutrients; Volume 8; Issue 10; Pages: 594 Nutrients, Vol 8, Iss 10, p 594 (2016) |
ISSN: | 2072-6643 |
Popis: | Folic acid intake has increased to high levels in many countries, raising concerns about possible adverse effects, including disturbances to energy and lipid metabolism. Our aim was to investigate the effects of excess folic acid (EFA) intake compared to adequate folic acid (AFA) intake on metabolic health in a rodent model. We conducted these investigations in the setting of either a 15% energy low fat (LF) diet or 60% energy high fat (HF) diet. There was no difference in weight gain, fat mass, or glucose tolerance in EFA-fed rats compared to AFA-fed rats when they were fed a LF diet. However, rats fed EFA in combination with a HF diet had significantly greater weight gain and fat mass compared to rats fed AFA (p < 0.05). Gene expression analysis showed increased mRNA levels of peroxisome proliferator-activated receptor γ (PPARγ) and some of its target genes in adipose tissue of high fat-excess folic acid (HF-EFA) fed rats. Inflammation was increased in HF-EFA fed rats, associated with impaired glucose tolerance compared to high fat-adequate folic acid (HF-AFA) fed rats (p < 0.05). In addition, folic acid induced PPARγ expression and triglyceride accumulation in 3T3-L1 cells. Our results suggest that excess folic acid may exacerbate weight gain, fat accumulation, and inflammation caused by consumption of a HF diet. |
Databáze: | OpenAIRE |
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