Localization of Cells Containing Transforming Growth Factor- α Precursor Immunoreactivity in the Basal Ganglia of the Adult Rat Brain
| Autor: | James H. Fallon, Sandra E. Loughlin, Larry E. Gentry, Daniel R. Twardzik, Casey M. Annis |
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| Rok vydání: | 1990 |
| Předmět: |
Male
medicine.medical_specialty External capsule Clinical Biochemistry Substantia nigra Striatum Biology Globus Pallidus Basal Ganglia Ventral pallidum Endocrinology Internal medicine Basal ganglia medicine Animals Tissue Distribution Protein Precursors Immunoperoxidase Rats Inbred Strains Cell Biology Transforming Growth Factor alpha Immunohistochemistry Molecular biology Corpus Striatum Rats Globus pallidus nervous system Astrocytes Transforming Growth Factors Forebrain Female |
| Zdroj: | Growth Factors. 2:241-250 |
| ISSN: | 1029-2292 0897-7194 |
| DOI: | 10.3109/08977199009071510 |
| Popis: | Transforming growth factor-alpha-like immunoreactivity (TGF-alpha-ir) was visualized in the adult rat forebrain using three antisera directed against carboxyterminal sequences in the TGF-alpha precursor. Using immunoperoxidase and immunofluorescence techniques with all three antisera, TGF-alpha-ir was found to be present in a subpopulation of astrocytes in the forebrain. Striatal and pallidal regions of the basal ganglia were studied in detail. In the striatum, there was an uneven distribution of astrocytes containing TGF-alpha-ir, with the greatest number in the dorsal medial third of the caudate-putamen and the overlying corpus callosum/external capsule. In addition, the region of the caudate-putamen bordering the globus pallidus contained numerous clusters of TGF-alpha-ir astrocytes. The globus pallidus itself contained numerous and more evenly distributed TGF-alpha-ir astrocytes. Other pallidal structures--including the ventral pallidum, entopeduncular nucleus, and substantia nigra pars reticulata--contained moderate numbers of TGF-alpha-ir astrocytes. These results suggest that TGF-alpha precursor is present and, perhaps, synthesized in astrocytes. A related growth factor, epidermal growth factor (EGF), has also been reported to be present in pallidal regions of rat brain. Therefore, the TGF-alpha/EGF family of trophic factors may play a role in the function of the central nervous system. |
| Databáze: | OpenAIRE |
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