Purification of an LHI-RC-complex of Rhodospirillum rubrum by solubilization of chromatophores with a short-chain lecithin

Autor:	Robin Ghosh, Janine Kessi, Reinhard Bachofen
Rok vydání:	1995
Předmět:	food.ingredient Chromatography Rhodospirillum rubrum Two step Cell Biology Plant Science General Medicine Large range Biology biology.organism_classification Biochemistry Lecithin Chromatophore food Solubilization RC complex Absorption (chemistry)
Zdroj:	Photosynthesis research. 46(1-2)
ISSN:	0166-8595
Popis:	Chromatophores from Rhodospirillum rubrum were solubilized using the detergent 1,2-diheptanoyl-sn-phosphatidylcholine (DHPC). The solubilization curves are sigmoidal reaching a plateau at a detergent/protein ratio of 2–3 μmol/mg corresponding to 75–90% solubilized protein. The BChl-binding proteins are stable over a large range of DHPC/protein ratios. A complex of BChl-binding proteins containing both LHI- and RC-polypeptides (LHI-RC-complex) was purified using a two step procedure. RC photochemical activity as well as absorption and near-IR CD spectra showed the complex to be active and stable after purification in presence of DHPC.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d234f3af0d22fb02150f834e003c8b2d https://pubmed.ncbi.nlm.nih.gov/24301603 Zobrazit plný text záznamu Full text from SpringerLink