Evaluation of antimicrobial effect of azadirachtin plant extract (Soluneem ™) on commonly found root canal pathogenic microorganisms (viz. Enterococcus faecalis) in primary teeth: A microbiological study
| Autor: | Krishna Trivedi, Shanal Shah, Prashant Choudhary, Shameer Mohammad, Karthik Venkataraghavan, Shalin Shah |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Limonins Veterinary medicine Root canal Microorganism sodium hypochlorite Indicator Dilution Techniques Microbial Sensitivity Tests Soluneem ™ Enterococcus faecalis Microbiology 03 medical and health sciences chemistry.chemical_compound Anti-Infective Agents Antimicrobial effect medicine Humans General Dentistry 030109 nutrition & dietetics Minimum bactericidal concentration Azadirachta biology Dose-Response Relationship Drug Root Canal Irrigants business.industry Plant Extracts Antimicrobial biology.organism_classification macrobroth dilution lcsh:RK1-715 medicine.anatomical_structure Azadirachtin chemistry Sodium hypochlorite lcsh:Dentistry Seeds Antimicrobial efficacy Dental Pulp Cavity business |
| Zdroj: | Journal of Indian Society of Pedodontics and Preventive Dentistry, Vol 34, Iss 3, Pp 210-216 (2016) |
| ISSN: | 1998-3905 0970-4388 |
| Popis: | Aim: The aim of this study is to evaluate the antimicrobial activity of Soluneem ™ when used as an irrigating solution along with other commonly used irrigating solution sodium hypochlorite (NaOCl) against Enterococcus faecalis. Materials and Methods: Microorganism used in this study was E. faecalis (Microbial Type Culture Collection 439). Test substance used was Soluneem ™, which was obtained from Vittal Mallya Scientific Research Foundation (VMSRF), Bengaluru. This study was conducted in a microbiology laboratory (Biocare Research India Pvt., Ltd. Laboratory, Ahmedabad, Gujarat) to evaluate the antimicrobial effect of Soluneem ™ (Azadirachtin) on E. faecalis. Antimicrobial activity testing was performed using the macrobroth dilution method according to the Clinical Laboratory Standards Institute guidelines. All determinations were performed thrice. Results: Minimum bactericidal concentration (MBC) was seen as 2.6% for Soluneem ™ while the same was seen at 0.1% for NaOCl. Independent sample t-test was carried out to compare the MBC of Soluneem ™ and NaOCl, which showed that there was no statistically significant difference between them, i.e., 2.6% Soluneem ™ was as effective as 0.1% NaOCl. Conclusion: Soluneem ™ showed antimicrobial activity against E. faecalis at various concentrations. It was also found that the efficacy of Soluneem ™ at 2.6% concentration and above was relatively similar to that of gold standard irrigating solution (NaOCl) on inhibition of E. faecalis. |
| Databáze: | OpenAIRE |
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