Common Grounds for Family Maladies

Autor: Konrad Oexle, Juliane Winkelmann
Rok vydání: 2018
Předmět:
Zdroj: Neuron 98, 671-672 (2018)
ISSN: 0896-6273
DOI: 10.1016/j.neuron.2018.05.006
Popis: Flatworms of the species Schmidtea mediterranea are immortal-adult animals contain a large pool of pluripotent stem cells that continuously differentiate into all adult cell types. Therefore, single-cell transcriptome profiling of adult animals should reveal mature and progenitor cells. By combining perturbation experiments, gene expression analysis, a computational method that predicts future cell states from transcriptional changes, and a lineage reconstruction method, we placed all major cell types onto a single lineage tree that connects all cells to a single stem cell compartment. We characterized gene expression changes during differentiation and discovered cell types important for regeneration. Our results demonstrate the importance of single-cell transcriptome analysis for mapping and reconstructing fundamental processes of developmental and regenerative biology at high resolution.
Databáze: OpenAIRE