Umbilical Cord Wharton’s Jelly Repeated Culture System: A New Device and Method for Obtaining Abundant Mesenchymal Stem Cells for Bone Tissue Engineering

Autor: Jianzhong Xu, Tianyong Hou, Junchao Xing, Zhao Xie, Zhiqiang Li, Moyuan Deng, Huiyong Jin, Xuehui Wu, Zhengqi Chang
Rok vydání: 2014
Předmět:
Cellular differentiation
Orthopedic Surgery
Cell Culture Techniques
lcsh:Medicine
Umbilical cord
Umbilical Cord
Mice
Immunophenotyping
Tissue engineering
Animal Cells
Osteogenesis
Molecular Cell Biology
Wharton's jelly
Medicine and Health Sciences
lcsh:Science
Musculoskeletal System
Cells
Cultured
Mice
Inbred BALB C
Multidisciplinary
medicine.diagnostic_test
Stem Cells
Cell Differentiation
medicine.anatomical_structure
Connective Tissue
Anatomy
Cellular Types
Research Article
Biotechnology
Biomedical Engineering
Mice
Nude
Bioengineering
Surgical and Invasive Medical Procedures
Biology
Flow cytometry
Andrology
Musculoskeletal System Procedures
medicine
Animals
Humans
Bone
Cell Proliferation
Tissue Engineering
lcsh:R
Mesenchymal stem cell
Biology and Life Sciences
Mesenchymal Stem Cells
Cell Biology
Biological Tissue
Cell culture
Immunology
lcsh:Q
Zdroj: PLoS ONE
PLoS ONE, Vol 9, Iss 10, p e110764 (2014)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0110764
Popis: To date, various types of cells for seeding regenerative scaffolds have been used for bone tissue engineering. Among seed cells, the mesenchymal stem cells derived from human umbilical cord Wharton's jelly (hUCMSCs) represent a promising candidate and hold potential for bone tissue engineering due to the the lack of ethical controversies, accessibility, sourced by non-invasive procedures for donors, a reduced risk of contamination, osteogenic differentiation capacities, and higher immunomodulatory capacity. However, the current culture methods are somewhat complicated and inefficient and often fail to make the best use of the umbilical cord (UC) tissues. Moreover, these culture processes cannot be performed on a large scale and under strict quality control. As a result, only a small quantity of cells can be harvested using the current culture methods. To solve these problems, we designed and evaluated an UC Wharton's jelly repeated culture device. Using this device, hUCMSCs were obtained from the repeated cultures and their quantities and biological characteristics were compared. We found that using our culture device, which retained all tissue blocks on the bottom of the dish, the total number of obtained cells increased 15-20 times, and the time required for the primary passage was reduced. Moreover, cells harvested from the repeated cultures exhibited no significant difference in their immunophenotype, potential for multilineage differentiation, or proliferative, osteoinductive capacities, and final osteogenesis. The application of the repeated culture frame (RCF) not only made full use of the Wharton's jelly but also simplified and specified the culture process, and thus, the culture efficiency was significantly improved. In summary, abundant hUCMSCs of dependable quality can be acquired using the RCF.
Databáze: OpenAIRE
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