Human interleukin 7: molecular cloning and growth factor activity on human and murine B-lineage cells
| Autor: | Raymond G. Goodwin, Kathryn Hjerrild, Rita Jerzy, David Cosman, Anthony E. Namen, William Clevenger, Stephen D. Lupton, Steve Gillis, Ann E. Schmierer |
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| Rok vydání: | 1989 |
| Předmět: |
Molecular Sequence Data
Biology Lymphocyte Activation Mice Interleukin 20 Interleukin 26 Sequence Homology Nucleic Acid Complementary DNA Escherichia coli Animals Humans Amino Acid Sequence Cloning Molecular Growth Substances Interleukin 12 receptor beta 1 subunit Interleukin 3 B-Lymphocytes Multidisciplinary Base Sequence Interleukin-7 Interleukins Interleukin Molecular biology Recombinant Proteins Genes Interleukin 15 Interleukin 19 Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 86:302-306 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.86.1.302 |
| Popis: | A cDNA encoding biologically active human interleukin 7 was isolated by hybridization with the homologous murine clone. Nucleotide sequence analysis indicated that this cDNA was capable of encoding a protein of 177 amino acids with a signal sequence of 25 amino acids and a calculated mass of 17.4 kDa for the mature protein. Recombinant human interleukin 7 stimulated the proliferation of murine pre-B cells and was active on cells harvested from human bone marrow that are enriched for B-lineage progenitor cells. Analysis of RNA by blot hybridization demonstrated the presence of two size classes of interleukin 7 mRNA in human splenic and thymic tissue. |
| Databáze: | OpenAIRE |
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