Mild Moxibustion Decreases the Expression of Prokineticin 2 and Prokineticin Receptor 2 in the Colon and Spinal Cord of Rats with Irritable Bowel Syndrome
| Autor: | Shi-min Liu, Wen Liao, Cili Zhou, Luyi Wu, Xiaomei Wang, Yin Shi, Huang Renjia, Ji-Meng Zhao, Jue Hong, Huangan Wu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Pathology
medicine.medical_specialty Article Subject business.industry medicine.medical_treatment Withdrawal reflex Prokineticin receptor 2 Moxibustion lcsh:Other systems of medicine medicine.disease Spinal cord lcsh:RZ201-999 Gastroenterology medicine.anatomical_structure Complementary and alternative medicine Internal medicine Hyperalgesia medicine Immunohistochemistry medicine.symptom Receptor business Irritable bowel syndrome Research Article |
| Zdroj: | Evidence-Based Complementary and Alternative Medicine, Vol 2014 (2014) Evidence-based Complementary and Alternative Medicine : eCAM |
| ISSN: | 1741-4288 |
| Popis: | It has been proven that prokineticin 2 (PK2) and its receptor PKR2 play an important role in hyperalgesia, while mild moxibustion can relieve visceral hypersensitivity in a rat model of irritable bowel syndrome (IBS). The goal of the present study was to determine the effects of mild moxibustion on the expression of PK2 and PKR2 in colon and spinal cord in IBS rat model, which was induced by colorectal distension using inflatable balloons. After mild moxibustion treatment, abdominal withdrawal reflex (AWR) scores were assessed by colorectal distension; protein and mRNA expression of PK2 and PKR2 in rat colon and spinal cord was determined by immunohistochemistry and fluorescence quantitative PCR. Compared with normal rats, the AWR scores of rats and the expressions of PK2/PKR2 proteins and mRNAs in colon and spinal cord tissue were significantly increased in the model group; compared with the model group, the AWR scores of rats and the expressions of PK2/PKR2 proteins and mRNAs in colon and spinal cord tissue were significantly decreased in the mild moxibustion group. These findings suggest that the analgesia effect of mild moxibustion may be associated with the reduction of the abnormally increased expression of the PK2/PKR2 proteins and mRNAs in the colon and spinal cord. |
| Databáze: | OpenAIRE |
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