Isolation and characterization of human interleukin-10-secreting T cells from peripheral blood
| Autor: | Rachel E. Protheroe, David C. Wraith, Andrew Herman, John D. M. Campbell, Catherine A Sabatos-Peyton, Graziella Mazza |
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| Rok vydání: | 2009 |
| Předmět: |
CD4-Positive T-Lymphocytes
Immunology Cell Culture Techniques Cell Separation Biology Lymphocyte Activation Article Immunophenotyping Interleukin 21 Antigens CD Immunology and Allergy Cytotoxic T cell Humans CTLA-4 Antigen IL-2 receptor Antigens Antigen-presenting cell Interleukin 3 Cell Proliferation Forkhead Transcription Factors General Medicine Bystander Effect Natural killer T cell Flow Cytometry Molecular biology Interleukin-10 Interleukin 10 Blood Circulation Interleukin 12 |
| Zdroj: | Human immunology. 71(3) |
| ISSN: | 1879-1166 |
| Popis: | Recent studies have expanded our understanding of the role of the anti-inflammatory cytokine interleukin (IL)-10, produced by multiple lineages of both human and murine T cells, in regulating the immune response. Here, we demonstrate that the small percentage of circulating CD4(+) T cells that secrete IL-10 can be isolated from human peripheral blood and, importantly, we have optimized a protocol to expand these cells in both antigen-specific and polyclonal manners. Expanded CD4(+)IL-10(+) T cells abrogate proliferation and T helper (Th) 1-like cytokine production in an antigen-specific manner, and to a lesser extent exhibit bystander suppressive capacity. CD4(+)IL-10(+) T cells are suppressive in a cell contact-dependent way, though they do not require secretion of IL-10 for their suppressive role in vitro. CD4(+)IL-10(+) T cells have an activated phenotype, with high expression of CD25, CD69, and cytotoxic T-lymphocyte antigen-4, and are largely FoxP3 negative. This novel method for the isolation and expansion of suppressive IL-10-secreting T cells has important implications both for further research and clinical therapeutic development. |
| Databáze: | OpenAIRE |
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