Inducible nitric oxide synthase in duodenum of children with Giardia lamblia infection
| Autor: | Małgorzata Mokrzycka, Barbara Wiszniewska, Agnieszka Kolasa, Anita Kosierkiewicz |
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| Rok vydání: | 2010 |
| Předmět: |
Giardiasis
Pathology medicine.medical_specialty Histology Malabsorption Adolescent Duodenum Biopsy Connective tissue Nitric Oxide Synthase Type II Biology medicine.disease_cause Pathology and Forensic Medicine fluids and secretions parasitic diseases medicine Giardia lamblia Animals Humans lcsh:QH573-671 Intestinal Mucosa Child Gastrointestinal tract Lamina propria lcsh:Cytology General Medicine medicine.disease Intestinal epithelium Mucus medicine.anatomical_structure Child Preschool |
| Zdroj: | Folia Histochemica et Cytobiologica, Vol 48, Iss 2, Pp 191-196 (2010) |
| ISSN: | 1897-5631 |
| Popis: | The investigation were performed on children with Giardia lamblia infection, diagnosed on the basis of positive stool tests for Giardia antigen (Elisa) or by microscopical detection of trophozoites in duodenal fluid. In duodenal biopsies morphological studies and immunohistochemical reaction for inducible nitric oxide synthase (iNOS) were performed. The control group was made up of duodenal tissue of children with excluded giardiasis and inflammation of the upper part of gastrointestinal tract. The duodenal biopsies from children without Giardia lamblia infection were found to have a high immunoreactivity for iNOS in enterocytes, the cells of intestinal crypts, endothelial cells of vessels and connective tissue cells of lamina propria. In children with giardiasis: in some biopsies the expression of iNOS was as high as in control group, in others was weaker detectable and the shortening of intestinal villi was seen. There were also duodenal biopsies with the lack of immunoreactivity for iNOS, with shorter villi and a large amount of mucus in the intestinal epithelium. Beside of goblet cells, also enterocytes were loaded with mucus. The pathological changes may cause malabsorption and also may have a negative influence on the defense of the intestinal wall against Giardia lamblia infection. The different morphological and immunohistochemical results in the duodenum of children with giardiasis can elucidate a variety of clinical symptoms from asymptomatic to severe infection. |
| Databáze: | OpenAIRE |
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