Expression of wild-type estrogen receptor beta protein in human breast cancer: specific correlation with HER2/neu overexpression
| Autor: | Yasuyo Ohi, Tetsuya Takahama, Yoshiaki Rai, Yoshiatsu Sagara, Yoshihisa Umekita, Masakazu Souda, Hiroki Yoshida |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
medicine.medical_specialty Pathology Receptor ErbB-2 Estrogen receptor Breast Neoplasms Antibodies Monoclonal Humanized HER2/neu Pathology and Forensic Medicine Immunoenzyme Techniques Internal medicine Progesterone receptor medicine Biomarkers Tumor Estrogen Receptor beta Humans skin and connective tissue diseases Mammary Glands Human Estrogen receptor beta Aged Neoplasm Staging Aged 80 and over Cell Nucleus biology Carcinoma Ductal Breast Cancer Antibodies Monoclonal General Medicine Middle Aged Trastuzumab medicine.disease Endocrinology Cancer cell Cancer research biology.protein Immunohistochemistry Female Reagent Kits Diagnostic Antibody Receptors Progesterone hormones hormone substitutes and hormone antagonists |
| Zdroj: | Pathology international. 56(8) |
| ISSN: | 1320-5463 |
| Popis: | Expression of estrogen receptor beta (ERbeta) protein in human breast cancer and correlation with clinicopathological factors have been reported by many investigators, but many of them used ERbeta antibodies that react with both wild-type ERbeta (ERbetawt) and splicing variant isoform. Therefore, the frequency and correlation with clinicopathological factors of ERbetawt expression remain to be established. In the present study a monoclonal antibody EMR02, specific for ERbetawt, was used in formalin-fixed paraffin-embedded sections from 225 female primary breast cancer patients diagnosed as having invasive ductal carcinoma. Expression of ERalpha, progesterone receptor (PgR) and HER2/neu were also investigated by immunohistochemistry. For ERbetawt, ERalpha and PgR, positivity was defined as nuclear staining in >10% of the cancer cells. HER2/neu overexpression was defined as a Hercep test score 3+. Positivity for ERbetawt, ERalpha, PgR and HER2/neu overexpression was 55%, 74%, 61% and 25%, respectively. The expression of ERbetawt had a positive correlation with ERalpha (P=0.018) and PgR (P=0.02). There was significant positive correlation between ERbetawt expression and HER2/neu overexpression (P |
| Databáze: | OpenAIRE |
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