Speciation of human microsporidia by polymerase chain reaction single-strand conformation polymorphism
| Autor: | Daniel P. Fedorko, Rosario M. Delgado, Nancy A. Nelson, Elizabeth S. Didier, Andrew M. Hruszkewycz, D. C. Bertucci |
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| Rok vydání: | 2001 |
| Předmět: |
Genotype
Proteolipids Nerve Tissue Proteins Microsporidiosis Polymerase Chain Reaction law.invention Feces fluids and secretions law Virology parasitic diseases medicine Animals Humans Enterocytozoon bieneusi Encephalitozoon cuniculi Polymerase chain reaction Polymorphism Single-Stranded Conformational DNA Primers Genetics Electrophoresis Agar Gel biology Myelin and Lymphocyte-Associated Proteolipid Proteins fungi Gene Amplification virus diseases Membrane Proteins Reproducibility of Results Single-strand conformation polymorphism DNA Protozoan biology.organism_classification medicine.disease Infectious Diseases Microsporidia Parasitology Primer (molecular biology) |
| Zdroj: | The American journal of tropical medicine and hygiene. 65(4) |
| ISSN: | 0002-9637 |
| Popis: | We describe the application of single-strand conformation polymorphism (SSCP) analysis to the speciation of human microsporidia after polymerase chain reaction (PCR) amplification with the panmicrosporidian primers PMP1 and PMP2. We compared the DNA extracted and amplified from different genotypes or isolates of Enterocytozoon bieneusi, Encephalitozoon cuniculi, E. hellem, and E. intestinalis plus an isolate of Vittaforma corneae. The PCR-SSCP, when performed at 20 degrees C, generated 2 bands in distinctive, reproducible patterns in polyacrylamide gels for each species of microsporidia tested, regardless of genotype or isolate. We found PCR-SSCP to be an easy and reproducible method for speciation of human microsporidia when the primer pair PMP1 and PMP2 is used. |
| Databáze: | OpenAIRE |
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