Cytoglobin is expressed in the vasculature and regulates cell respiration and proliferation via nitric oxide dioxygenation
| Autor: | David Jourd'heuil, Frances L. Jourd'heuil, Katharine E. Halligan |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Vascular smooth muscle
Cellular respiration Myocytes Smooth Muscle Biology Nitric Oxide Biochemistry Muscle Smooth Vascular Nitric oxide Small hairpin RNA chemistry.chemical_compound Mice Oxygen Consumption Animals Globin Molecular Biology Aorta Cell Proliferation Nitrates Cell growth Cytoglobin Mechanisms of Signal Transduction Cell Biology Fibroblasts Cell biology Globins Rats chemistry Gene Expression Regulation Connective Tissue NIH 3T3 Cells Intracellular |
| Zdroj: | The Journal of biological chemistry. 284(13) |
| ISSN: | 0021-9258 |
| Popis: | Disposition of the second messenger nitric oxide (NO) in mammalian tissues occurs through multiple pathways including dioxygenation by erythrocyte hemoglobin and red muscle myoglobin. Metabolism by a putative NO dioxygenase activity in non-striated tissues has also been postulated, but the exact nature of this activity is unknown. In the present study, we tested the hypothesis that cytoglobin, a newly discovered hexacoordinated globin, participates in cell-mediated NO consumption. Stable expression of small hairpin RNA targeting cytoglobin in fibroblasts resulted in decreased NO consumption and intracellular nitrate production. These cells were more sensitive to NO-induced inhibition of cell respiration and proliferation, which could be restored by re-expression of human cytoglobin. We also demonstrated cytoglobin expression in adventitial fibroblasts as well as vascular smooth muscle cells from various species including human and found that cytoglobin was expressed in the adventitia and media of intact rat aorta. These results indicate that cytoglobin contributes to cell-mediated NO dioxygenation and represents an important NO sink in the vascular wall. |
| Databáze: | OpenAIRE |
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