DETECTION OF SPECIFIC MURINE ANTI-TUMOUR IMMUNITY AND SERUM FACTORS BY THE INDIRECT E-ROSETTE AUGMENTATION ASSAY
| Autor: | Phillips Rg, Halliday Wj |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Rosette Formation
Fibrosarcoma Anti tumour immunity Clinical Biochemistry Immunology E rosettes Binding Competitive Epitopes Mice chemistry.chemical_compound Antigen Antigens Neoplasm Animals Humans Immunity Cellular Chemistry Lymphokine Blood Proteins Neoplasms Experimental Cell Biology General Medicine Molecular biology Lipoproteins LDL Antigenic Specificity Methylcholanthrene Mice Inbred CBA |
| Zdroj: | Australian Journal of Experimental Biology and Medical Science. 61:173-180 |
| ISSN: | 0004-945X |
| Popis: | The spontaneous interaction of sheep erythrocytes and human lymphocytes results in the formation of aggregates known as E-rosettes. The antigen reactivity of murine T-lymphocytes can be detected indirectly by the production of a lymphokine that augments the E-rosette-forming ability of human lymphocytes. An indirect E-rosette augmentation (ERA) assay was used to study cellular reactivity and tumour-related serum reactions in mice bearing methylcholanthrene (MCA)-induced tumours. The assay was used to demonstrate the antigenic specificity of two tumours and to detect blocking factors in tumour-bearer serum. Blocking factors were shown to be tumour-specific and to act on lymphocytes at an early stage before exposure to antigen. |
| Databáze: | OpenAIRE |
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