A somatic cell hybrid panel for pig regional gene mapping characterized by molecular cytogenetics
| Autor: | Joël Gellin, A. Mairal, Martine Yerle, Philippe Pinton, David J. Milan, Annie Robic, C. Dubut-Fontana, Y. Lahbib-Mansais, Juliette Riquet, G. Echard |
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| Přispěvatelé: | Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées |
| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
Genetic Markers
Male clone (Java method) DNA Complementary Swine Somatic cell [SDV]Life Sciences [q-bio] Molecular Sequence Data Hybrid Cells Biology Polymerase Chain Reaction Cell Line Molecular cytogenetics 03 medical and health sciences 0302 clinical medicine Gene mapping Cricetinae Genetics Animals Molecular Biology Metaphase Gene In Situ Hybridization Fluorescence Genetics (clinical) 030304 developmental biology 0303 health sciences Base Sequence Chromosome Mapping Chromosome 030220 oncology & carcinogenesis Microsatellite Female DNA Probes |
| Zdroj: | Cytogenetics and Cell Genetics Cytogenetics and Cell Genetics, S Karger AG, 1996, 73, pp.194-202 |
| ISSN: | 0301-0171 1421-9816 |
| Popis: | A panel of 27 pig × rodent somatic cell hybrids was produced and characterized cytogenetically. The first step of this study consisted of hybridizing a SINE probe to GTG-banded metaphases of each hybrid clone in order to count and identify the normal pig chromosomes and to detect rearranged ones. The second step consisted of using the DNA of each clone as a probe after pIRS-PCR (porcine interspersed repetitive sequence-polymerase chain reaction) amplification to highly enrich it in pig sequences. These probes, hybridized to normal pig metaphase chromosomes, enabled the identification of the complete porcine complement in the hybrid lines. Whole chromosomes and fragments were characterized quickly and precisely, and results were compared. In addition to this cytogenetic characterization, molecular verification was also carried out by using primers specific to six microsatellites and to one gene previously mapped to pig chromosomes. The results obtained allow us to conclude that we have produced a panel that is informative for all porcine chromosomes. This panel constitutes a highly efficient tool to establish not only assignments of genes and markers but also regional localizations on pig chromosomes. |
| Databáze: | OpenAIRE |
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