MiR-151a is involved in the pathogenesis of atopic dermatitis by regulating interleukin-12 receptor β2
| Autor: | Wei Zhang, Xia Dou, Zhang Lijuan, Jie Zhang, Bo Yu, Xiaofan Chen, Xiao-Jian Jia, Yong Shao |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Untranslated region Male Adolescent Down-Regulation Dermatology Biology Biochemistry Jurkat cells Dermatitis Atopic Pathogenesis 03 medical and health sciences Interferon-gamma Jurkat Cells Young Adult microRNA medicine Humans Interleukin-12 Receptor beta 2 Subunit Phytohemagglutinins Child Molecular Biology Pathological Gene 3' Untranslated Regions Infant Newborn Infant Atopic dermatitis medicine.disease Interleukin-12 body regions 030104 developmental biology Gene Expression Regulation Case-Control Studies Child Preschool Interleukin-12 receptor Immunology Interleukin-2 Female Biomarkers |
| Zdroj: | Experimental dermatology. 27(4) |
| ISSN: | 1600-0625 |
| Popis: | MicroRNAs (miRNAs) have been reported to circulate in the blood in a highly stable and cell-free form. Dysregulated expression of miRNAs has been detected in various pathological conditions including atopic dermatitis. In our study, human blood plasma miRNAs were identified by high-throughput sequencing and compared among patients of atopic dermatitis and healthy controls. We found that miR-151a was differentially expressed in the plasma of atopic dermatitis patients. MiR-151a regulates the expression of IL12RB2 by targeting two loci in the 3' untranslated region of the Il12rb2 gene. Moreover, IL12RB2 was remarkably downregulated in Jurkat cells overexpressing miR-151a. Jurkat cells treated with phytohemagglutinin also showed reduced expression of IFN-γ, interleukin-2 (IL-2) and IL-12. Together, these results suggest that miR-151a is involved in the pathogenesis of atopic dermatitis by regulating IL12RB2. |
| Databáze: | OpenAIRE |
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