Structure and expression of mitochondrial citrate synthases from higher plants
| Autor: | Lothar Willmitzer, Bernd Müller-Röber, U La Cognata, Volker Landschütze |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
DNA
Complementary Physiology Protein Conformation Nicotiana tabacum Molecular Sequence Data Plant Science Citrate (si)-Synthase Crystallography X-Ray Isozyme Citrate synthase Amino Acid Sequence Cloning Molecular Peptide sequence Phylogeny chemistry.chemical_classification biology Sequence Homology Amino Acid fungi Nucleic acid sequence food and beverages Cell Biology General Medicine Plants biology.organism_classification Amino acid Mitochondria Citric acid cycle Biochemistry chemistry biology.protein |
| Zdroj: | Plantcell physiology. 37(7) |
| ISSN: | 0032-0781 |
| Popis: | Mitochondrial citrate synthase (EC 4.1.3.7) represents the first enzyme of the tricarboxylic acid cycle, catalyzing the condensation of acetyl-CoA and oxaloacetate, finally yielding citrate and CoA. We report here the isolation of cDNA clones encoding citrate synthase from Nicotiana tabacum, Beta vulgaris and Populus. Nucleotide and deduced amino acid sequences were compared with previously published sequences of mitochondrial citrate synthases from Arabidopsis thaliana and potato, as well as with the sequence of glyoxysomal citrate synthase from pumpkin. Homologies between the various plant mitochondrial enzymes were in the range from 77.2% (potato vs. Arabidopsis) to 94.2% (potato vs. tobacco) on the nucleotide level (coding regions only), and in the range from 70.1% to 90.4% (potato vs. Arabidopsis, and potato vs. tobacco, respectively) on the amino acid level. Identities of the mitochondrial isozymes to the pumpkin glyoxysomal enzyme were below 30% on the nucleotide and amino acid level. In Northern blot experiments citrate synthase mRNA was detected in all tissues analyzed. However, levels of expression showed tissue dependency despite the fact that citrate synthase is usually considered a house-keeping enzyme. Whether these different levels of expression reflect tissue-specific variations with respect to basic metabolism awaits further analysis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|