TH17-Based Vaccine Design for Prevention of Streptococcus pneumoniae Colonization
| Autor: | Richard Malley, Jessica Flechtner, Paulo Gouveia, Mark R. Alderson, Todd Gierahn, Darren E. Higgins, Kristin Moffitt, Ying-Jie Lu |
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| Rok vydání: | 2011 |
| Předmět: |
Cancer Research
medicine.disease_cause Microbiology Pneumococcal Infections Pneumococcal Vaccines Mice 03 medical and health sciences Immune system Antigen Immunity Virology Immunology and Microbiology(all) Streptococcus pneumoniae medicine Animals Humans Molecular Biology 030304 developmental biology Antigens Bacterial Mice Inbred BALB C 0303 health sciences biology 030306 microbiology Interleukin-17 medicine.disease 3. Good health Mice Inbred C57BL Pneumococcal infections Pneumococcal vaccine Immunization Immunology biology.protein Th17 Cells Parasitology Antibody |
| Zdroj: | Cell Host & Microbe. 9(2):158-165 |
| ISSN: | 1931-3128 |
| DOI: | 10.1016/j.chom.2011.01.007 |
| Popis: | SummaryStreptococcus pneumoniae is a leading cause of mortality in young children. While successful conjugate polysaccharide vaccines exist, a less expensive serotype-independent protein-based pneumococcal vaccine offers a major advancement for preventing life-threatening pneumococcal infections, particularly in developing nations. IL-17A-secreting CD4+ T cells (TH17) mediate resistance to mucosal colonization by multiple pathogens including S. pneumoniae. Screening an expression library containing >96% of predicted pneumococcal proteins, we identified antigens recognized by TH17 cells from mice immune to pneumococcal colonization. The identified antigens also elicited IL-17A secretion from colonized mouse splenocytes and human PBMCs suggesting that similar responses are primed during natural exposure. Immunization of two mouse strains with identified antigens provided protection from pneumococcal colonization that was significantly diminished in animals treated with blocking CD4 or IL-17A antibodies. This work demonstrates the potential of proteomic screening approaches to identify specific antigens for the design of subunit vaccines against mucosal pathogens via harnessingTH17-mediated immunity. |
| Databáze: | OpenAIRE |
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