Involvement of Inducible Nitric Oxide Synthase in Blood Flow Decrease in Vein Induced by Hen-Egg White Lysozyme
| Autor: | Emiko Iwaoka, Haruyasu Ueda, Hisae Oku, Yuko Ogawa, Kyoko Ishiguro, Haruki Okamura, Masaru Kunitomo |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Thromboxane Nitric Oxide Synthase Type II Pharmaceutical Science Prostacyclin Peptides Cyclic Veins Microcirculation Nitric oxide Mice Thromboxane A2 chemistry.chemical_compound Internal medicine Hypersensitivity medicine Animals Nitrobenzenes Pharmacology Sulfonamides biology General Medicine Epoprostenol Mice Inbred C57BL Nitric oxide synthase NG-Nitroarginine Methyl Ester Endocrinology chemistry Regional Blood Flow biology.protein Methacrylates Female Muramidase Cyclooxygenase Endothelin receptor medicine.drug |
| Zdroj: | Biological and Pharmaceutical Bulletin. 30:1324-1328 |
| ISSN: | 1347-5215 0918-6158 |
| DOI: | 10.1248/bpb.30.1324 |
| Popis: | Our in vivo assay system developed to search for allergy-preventive substances, assesses the blood flow decrease in tail vein microcirculation of mice subjected to sensitization with hen-egg white lysozyme (HEL). The blood flow decrease appears to be regulated by various factors such as nitric oxide (NO), thromboxane (TX) A(2), prostacyclin (PGI(2)) and endothelin (ET)-1 together with cyclooxygenase (COX)-1, COX-2, inducible nitric oxide synthase (iNOS), and constitutive nitric oxide synthase (cNOS). In this study, we examined in detail the roles of iNOS in this assay system using an iNOS knockout (KO) mouse. We found that the blood flow decrease in the HEL-sensitized iNOS KO mice was slightly weaker than that in their wild type (WT) mice. This blood flow decrease was not affected by a selective COX-1 inhibitor, a selective COX-2 inhibitor and a PGI(2) agonist unlike the case of the WT mice. However, it was inhibited by a nonselective NOS inhibitor, a specific TXA(2) synthase inhibitor and a specific ET-1 receptor blocker as in the case of the WT mice. The present results indicate that the blood flow decrease occurs via two pathways; one is an iNOS-independent response involving TXA(2) and ET-1, and the other is an iNOS-dependent response involving COX-1, COX-2 and PGI(2). cNOS appears to play some roles in the blood flow decrease and iNOS acts as an exacerbation factor. Our method using HEL-sensitized should be useful for searching for agents that can prevent allergy via new mechanisms. |
| Databáze: | OpenAIRE |
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