Long-term stability and age-dependence of six regulatory serum proteins

Autor: Menne, Felix, Schipke, Carola G, Clark, Christopher, Popp, Julius
Přispěvatelé: University of Zurich, Menne, Felix
Rok vydání: 2022
Předmět:
Zdroj: Biomarkers in Medicine. 16:511-521
ISSN: 1752-0371
1752-0363
DOI: 10.2217/bmm-2021-0518
Popis: Aim: The development of biomarker-based diagnostic procedures often relies on samples stored for several years. We aimed to investigate the influence of storage time and patient age on six neuroregulatory and immunoregulatory serum biomarkers. Materials & methods: We quantified six biomarkers in serum from 151 individuals using ELISA. Serum was stored at -80°C for up to 9.5 years. Results: When associating storage time with biomarker values, BDNF, VEGF-A and TGF-β1 showed a significant increase over time; IGF-1, MCP-1 and IL-18 did not. Associating participant age with biomarkers, only IL-18 in Alzheimer’s disease patients showed a significant increase. Conclusion: Storage time can influence results of biomarkers in human serum. This needs to be considered when assessing samples stored for several years.
Databáze: OpenAIRE
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