Matrix Metalloproteinase Release From Placental Explants of Pregnancies Complicated by Intrauterine Growth Restriction
| Autor: | Larry J. Guilbert, Sandra T. Davidge, Philip N. Baker, Crocker Ip, Shaila J. Merchant, Tansinda D |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Placenta Blotting Western Intrauterine growth restriction Biology Matrix metalloproteinase Extracellular matrix 03 medical and health sciences 0302 clinical medicine Western blot Pregnancy Fetal membrane Culture Techniques Internal medicine medicine Extracellular Humans Zymography Tissue Inhibitor of Metalloproteinase-2 Fetal Growth Retardation Tissue Inhibitor of Metalloproteinase-1 030219 obstetrics & reproductive medicine medicine.diagnostic_test Obstetrics and Gynecology medicine.disease Matrix Metalloproteinases Oxygen medicine.anatomical_structure Endocrinology Matrix Metalloproteinase 9 Matrix Metalloproteinase 2 Female 030217 neurology & neurosurgery |
| Zdroj: | Journal of the Society for Gynecologic Investigation. 11:97-103 |
| ISSN: | 1071-5576 |
| DOI: | 10.1016/j.jsgi.2003.08.005 |
| Popis: | There is evidence of impaired placental development in intrauterine growth restriction (IUGR). Matrix metalloproteinases (MMPs) are extracellular matrix-degrading enzymes that are released by placental cells during tissue remodeling processes. We hypothesized 1) that release of MMP-2 and -9 is decreased and/or release of tissue inhibitors of metalloproteinases (TIMPs) is increased from placental explants in pregnancies complicated by IUGR and 2) that oxygen levels affect such release.Placental villous explants from normal (n = 7) and IUGR (n = 7) pregnancies were cultured at high (20%) and low (3%) oxygen levels for 24 hours. Supernatants were analyzed for MMP-2 and MMP-9 by zymography and for TIMP-1 and -2 by western blot analysis.: At 20% oxygen there was significantly reduced MMP-2 (P.05) and TIMP-1 (P.01) release and a trend for decreased MMP-9 release (P = .07) in explants from IUGR pregnancies compared with normal pregnancies; however, there were no differences at 3% oxygen. TIMP-2 was below detectable levels in all samples. Although MMP-2 and TIMP-1 release was significantly reduced at 3% compared with 20% oxygen in explants from both normal (P.001; P.05) and IUGR (P.05) pregnancies, MMP-2 release changed less in IUGR compared with normal explant cultures. There were no significant effects of oxygen on MMP-9 release.Placental explants from IUGR pregnancies demonstrated reduced MMP-2, MMP-9, and TIMP-1 release compared with explants from normal pregnancies at high (20%) but not low (3%) oxygen. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink