Evaluating methods of mouse euthanasia on the oocyte quality: cervical dislocation versus isoflurane inhalation
| Autor: | Jeanne Perrin, Alain Botta, Blandine Courbiere, Audrey Roustan, Erica Lopez, Anais Berthelot-Ricou |
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| Rok vydání: | 2012 |
| Předmět: |
Animal Use Alternatives
Mice Inbred Strains Superovulation Animal Welfare Mice Ovarian Follicle Euthanasia Animal Administration Inhalation medicine Animals Spinal Cord Injuries Alternative methods Isoflurane General Veterinary Inhalation business.industry Metaphase ii Ovary Oocyte medicine.anatomical_structure Anesthesia Anesthetics Inhalation Cervical dislocation Cervical Vertebrae Oocytes Female Animal Science and Zoology Artifacts business Cervical vertebrae medicine.drug |
| Zdroj: | Laboratory Animals. 46:167-169 |
| ISSN: | 1758-1117 0023-6772 |
| Popis: | Cervical dislocation is a commonly used method of mouse euthanasia. Euthanasia by isoflurane inhalation is an alternative method which allows the sacrifice of several mice at the same time with an anaesthesia, in the aim to decrease pain and animal distress. The objective of our study was to assess the impact of these two methods of euthanasia on the quality of mouse oocytes. By administering gonadotropins, we induced a superovulation in CD1 female mice. Mice were randomly assigned to euthanasia with cervical dislocation and isoflurane inhalation. Oviducts were collected and excised to retrieve metaphase II oocytes. After microscopic examination, oocytes were classified into three groups: intact, fragmented/cleaved and atretic. Intact metaphase II oocytes were employed for biomedical research. A total of 1442 oocytes in the cervical dislocation group were compared with 1230 oocytes in the isoflurane group. In the cervical dislocation group, 93.1% of the oocytes were intact, versus 65.8% in the isoflurane group ( P ≤ 0.001). In light of these results, we conclude that cervical dislocation is the best method of mouse euthanasia for obtaining intact oocytes for biomedical research. |
| Databáze: | OpenAIRE |
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